Shareen H. Doak

TL;DR: Many of the engineered nanomaterials assessed were found to cause genotoxic responses, such as chromosomal fragmentation, DNA strand breakages, point mutations, oxidative DNA adducts and alterations in gene expression profiles.

TL;DR: Current studies are reviewed and discussed how SPION, with or without different surface coating, may cause cellular perturbations including modulation of actin cytoskeleton, alteration in gene expression profiles, disturbance in iron homeostasis and altered cellular responses such as activation of signalling pathways and impairment of cell cycle regulation.

TL;DR: The data show that higher NP concentrations (200 nM) reduce cell viability mostly through induction of reactive oxygen species, which was significantly induced at concentrations of 50 nM Au NPs or higher, and put forward the described protocol as a possible template for future cell-NP interaction studies under comparable and standardized conditions.

TL;DR: This report focuses on nanomaterial interactions with colorimetric and fluorometric dyes, components of cell culture growth medium and genotoxicity assay components, and the resultant consequences on test systems are demonstrated.

TL;DR: A strategic in vitro genotoxicity testing strategy for nanomaterials is recommended and an in vitro HPRT and micronucleus assays requirenanomaterial specific protocols are recommended.