Sheetal Uppal

TL;DR: Site-directed mutagenesis of FB10, a residue known to have a key role in the regulation of hexacoordination and ligand affinity, resulted in substantial effects on the rebinding kinetics, partly inhibiting ligand exit to the solvent, enhancing geminate rebinding and enabling ligand migration within the internal cavities.

TL;DR: Crystal structure of Arabidopsis class I hemoglobin (AHb1) is homologous to the related members with similar globin fold and heme pocket architecture and the presence of a novel long tunnel connecting the distal pockets of both the monomers might suggest cooperative ligand binding and support its role in NO scavenging.

TL;DR: Evolutionary aspects of the carotenoid cleavage oxygenase superfamily and their enzymology are discussed to deduce what insight can be obtained from their evolutionary conservation.

TL;DR: It is suggested that RPE65 is indeed a dynamically-regulated palmitoylated protein and thatPalmitoylation is necessary for regulating its membrane binding, and to perform its normal visual cycle function.

TL;DR: The successful attempt at engineering heme stability holds promise for the production of stable Hb-based blood substitute and provides a new perspective to the evolution of Hbs.