Stanley A. Hefta

TL;DR: Reverse protein to DNA matching identified proteins for 118 previously unidentified ORFs in the PPP database, and the database permits examination of many other subsets, such as 1274 proteins identified with three or more peptides.

TL;DR: One acute phase reactant, orosomucoid, was readily observed in all three experiments to dramatically increase in abundance, thereby supporting the hypothesis that it would be possible to isolate an adequate amount of protein from a patient, having normal renal function, to identify biological markers of a particular disease state using a variety of proteomics techniques.

TL;DR: A Student's t-test was used to distinguish those proteins that significantly and reproducibly changed between carbon sources from those that did not, and showed a large degree of concordance with results reported by other groups in similar transcriptional profiling and proteomic experiments.

TL;DR: In vitro proteins were identified and prioritized based on their analytical confidence as well as their relevance to atherosclerosis pathways, and seven families of proteins were of particular interest: fatty acid-binding proteins, chitinase-like enzymes, cyclophilins, cathepsins, proteoglycans, urokinase-type plasminogen activator receptor, and a macrophage scavenger receptor.

TL;DR: The article elaborates methods and techniques utilized during the three steps of biomarker discovery process and develops preliminary assays to confirm the bio-analytical measurements from the first step and qualify the marker(s) in pre-clinical models, to initiate future marker validation and development.