Stefanie Klima

TL;DR: Suggestions are made on how DNT NAMs may be assembled into an integrated approach to testing and assessment (IATA), and a vision is presented on how further NAM development may be guided by knowledge of signaling pathways necessary for brain development, DNT pathophysiology, and relevant adverse outcome pathways (AOP).

TL;DR: It is concluded that transcriptome data after prolonged chemical stress of differentiating cells mainly reflect the altered developmental stage of the model system and not the drug MoA, and suggests that brief exposures, followed by immediate analysis, are more suitable for information on immediate drug responses and the toxicity MoA.

TL;DR: The PeriTox test was developed as a first toxicological assay that harnesses the potential of human pluripotent stem cells to generate cell types/tissues that are not otherwise available for the prediction of human systemic organ toxicity.

TL;DR: Comparative data suggest that complementary in vitro tests can pick up a broad range of toxicants, and that multiple test results might help to predict organ specificity patterns.

TL;DR: Two test systems, based on human embryonic stem cells and neuroectodermal progenitor cells, which recapitulates early neural development and predicts early developmental neurotoxicity and epigenetic changes triggered by chemicals, are suitable for identifying toxicity biomarkers.