Steffen Goletz

TL;DR: In this article, a mass spectrometric sequencing strategy was developed to identify in vivo O-glycosylation sites on mucin-derived glycopeptides, where the mass of C- or N-terminal fragments registered for the mono-to pentasubstituted PAP20 indicated that GalNAc was linked to the peptide at Ser5,Thr6 (GSTA) and Thr14(VTSA) but contrary to previous in vitro glycosylations studies also at Thr19 and Ser15 located within the PDTR or

TL;DR: In this article, the authors describe a fast and reliable technique for generating large diversities of anti-iotypic single chain antibody fragments from non-immunized phagemid libraries using phage display.

TL;DR: PankoMab is a novel antibody, which binds specifically to this epitope and was designed to show the highest glycosylation dependency and the strongest additive binding effect when compared to other MUC1 antibodies, which renders it a superior antibody for in vivo diagnostics and various immunotherapeutic approaches.

TL;DR: A hypothesis is proposed which might help to solve the problem of tumor specificity in at least a subgroup of stem cell markers, and Glycosylation may provide the key.

TL;DR: Evidence is presented that the antigenicity of the single repeat toward a considerable number of antibodies to the DTR motif is greatly enhanced if it is glycosylated within this motif, and only in this position, and several lines of evidence suggest that Glycosylation with N-acetylgalactosamine is sufficient for the observed enhancement effect.