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Steffen Goletz
Researcher at Max Delbrück Center for Molecular Medicine
Publications - 117
Citations - 2015
Steffen Goletz is an academic researcher from Max Delbrück Center for Molecular Medicine. The author has contributed to research in topics: Antigen & Antibody. The author has an hindex of 23, co-authored 114 publications receiving 1902 citations. Previous affiliations of Steffen Goletz include Humboldt State University & Charité.
Papers
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Journal ArticleDOI
Localization of O-Glycosylation Sites on Glycopeptide Fragments from Lactation-associated MUC1 ALL PUTATIVE SITES WITHIN THE TANDEM REPEAT ARE GLYCOSYLATION TARGETS IN VIVO
Stefan Müller,Steffen Goletz,Nicolle H. Packer,Andrew A. Gooley,Alexander M. Lawson,Franz-Georg Hanisch +5 more
TL;DR: In this article, a mass spectrometric sequencing strategy was developed to identify in vivo O-glycosylation sites on mucin-derived glycopeptides, where the mass of C- or N-terminal fragments registered for the mono-to pentasubstituted PAP20 indicated that GalNAc was linked to the peptide at Ser5,Thr6 (GSTA) and Thr14(VTSA) but contrary to previous in vitro glycosylations studies also at Thr19 and Ser15 located within the PDTR or
Journal ArticleDOI
Selection of large diversities of antiidiotypic antibody fragments by phage display.
Steffen Goletz,Peter Christensen,Peter Kristensen,Dietmar Blohm,Ian M. Tomlinson,Greg Winter,Uwe Karsten +6 more
TL;DR: In this article, the authors describe a fast and reliable technique for generating large diversities of anti-iotypic single chain antibody fragments from non-immunized phagemid libraries using phage display.
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PankoMab: a potent new generation anti-tumour MUC1 antibody
Antje Danielczyk,Renate Stahn,Dorian Faulstich,Anja Löffler,Angela Märten,Uwe Karsten,Steffen Goletz +6 more
TL;DR: PankoMab is a novel antibody, which binds specifically to this epitope and was designed to show the highest glycosylation dependency and the strongest additive binding effect when compared to other MUC1 antibodies, which renders it a superior antibody for in vivo diagnostics and various immunotherapeutic approaches.
Journal ArticleDOI
What makes cancer stem cell markers different
Uwe Karsten,Steffen Goletz +1 more
TL;DR: A hypothesis is proposed which might help to solve the problem of tumor specificity in at least a subgroup of stem cell markers, and Glycosylation may provide the key.
Journal Article
Enhanced Binding of Antibodies to the DTR Motif of MUC1 Tandem Repeat Peptide Is Mediated by Site-specific Glycosylation
Uwe Karsten,Catherine Diotel,Gunther Klich,Hans Paulsen,Steffen Goletz,Stefan Müller,Franz-Georg Hanisch +6 more
TL;DR: Evidence is presented that the antigenicity of the single repeat toward a considerable number of antibodies to the DTR motif is greatly enhanced if it is glycosylated within this motif, and only in this position, and several lines of evidence suggest that Glycosylation with N-acetylgalactosamine is sufficient for the observed enhancement effect.