Stephan K. W. Dertinger

TL;DR: This work presents a passive method for mixing streams of steady pressure-driven flows in microchannels at low Reynolds number, and uses bas-relief structures on the floor of the channel that are easily fabricated with commonly used methods of planar lithography.

TL;DR: In this paper, the authors describe a simple, versatile method of generating gradients in composition in solution or on surfaces using microfluidic systems based on controlled diffusive mixing of species in solutions that are flowing laminarly, at low Reynolds number, inside a network of microchannels.

TL;DR: A device microfabricated by soft lithography and consisting of a network of microfluidic channels that can generate spatially and temporally controlled gradients of chemotactic factors is described, providing a robust method to investigate migratory cells under a variety of conditions not accessible to study by earlier techniques.

TL;DR: In this paper, the authors describe the generation of gradients having complex shapes in solution using microfluidic networks, which can be useful in both biological and nonbiological research.

TL;DR: This article demonstrates a generally applicable technique for the fabrication of substrate-bound gradients of proteins with complex shapes, using laminar flows in microchannels, and showed that axon specification is oriented in the direction of increasing surface density of laminin.