Taiping Chen

TL;DR: Genome-wide profiling of pluripotent cells and differentiated cells suggests global chromatin remodelling during differentiation, which results in a progressive transition from a fairly open chromatin configuration to a more compact state, rather than merely stabilizing the gene expression changes that are driven by developmental transcription factors.

TL;DR: It is shown that DNMT3L-deficient mouse embryonic stem cells (mESCs) exhibit downregulation ofDNMT3A, especially DNMT1A2, the predominant DNMT2A isoform in mESCs, and this work uncovers a role for DNMT4L in maintaining DNMT 3A stability, which contributes to the effect of DNMT5L on DNMT7A-dependent DNA methylation.

TL;DR: This chapter will discuss the mechanisms of DNA methylation and demethylation in mammals, focusing on biochemical and genetic studies of the major players involved in these processes.

TL;DR: It is shown that Dnmt3b3, a catalytically inactive DnMT3b isoform expressed in differentiated cells, positively regulates de novo methylation by DNmt3a and Dn mt3b with a preference for Dn MT3b, suggesting that these isoforms facilitate de noovo DNA methylation during embryonic development and in somatic cells.

TL;DR: At the manufacturers’ recommended input RNA levels, all the RNA-Seq library preparation protocols evaluated were suitable for distinguishing between experimental groups, and the TruSeq Stranded mRNA kit was universally applicable to studies focusing on protein-coding gene profiles.