Telmo Henriques

TL;DR: The results suggest that enhancer H3K4me1 provides only a minor contribution to the long-range coactivator function of Mll3/4, and downregulated genes exhibit reduced polymerase levels in gene bodies, but not at promoters, suggestive of pause-release defects.

TL;DR: It is demonstrated that transcription is a nearly universal feature of enhancers in Drosophila and mammalian cells and that nascent RNA sequencing strategies are optimal for identification of both enhancers and superenhancers.

TL;DR: It is demonstrated that paused elongation complexes can be remarkably stable, with half-lives exceeding 15 min at genes with inefficient pause release, and proposed that stable pausing of polymerase provides a temporal window of opportunity for recruitment of factors to modulate gene expression and that the nascent tssRNA represents an appealing target for these interactions.

TL;DR: It is demonstrated that m6Am is an evolutionarily conserved mRNA modification mediated by the Phosphorylated CTD Interacting Factor 1 (PCIF1), which catalyzes m6A methylation on 2-O-methylated adenine located at the 5' ends of mRNAs.

TL;DR: A key role is uncovered for NELF-mediated pausing in establishing the responsiveness of stem cells to developmental cues in mouse ESCs in the naive, ground state.