Themistoklis M. Tsarouchas

TL;DR: It is shown that inhibiting inflammation reduces and promoting it accelerates axonal regeneration in spinal-lesioned zebrafish larvae, indicating a pro-regenerative role of Tnf-α.

TL;DR: It is demonstrated that in a vertebrate of high regenerative capacity, Wnt/β-catenin signaling controls the composition of the lesion site extracellular matrix and Collagen XII is identified as a promoter of axonal regeneration.

TL;DR: It is demonstrated that within 48 h of a spinal lesion or specific genetic ablation of motor neurons at 72 hpf, the pMN domain reverts to motor neuron generation at the expense of oligodendrogenesis and is promoted by the innate immune system.

TL;DR: In this article, a rapid in-vivo phenotypic screen for macrophage-related genes that promote regeneration after spinal injury was conducted, using acute injection of synthetic RNA Oligo CRISPR guide RNAs that were pre-screened for high activity in vivo.

TL;DR: In this paper, the authors used single-cell RNA-seq of progenitor cells and macrophages, as well as cell-type-specific manipulations, to provide evidence for a direct signaling axis from specific lesion-activated macrophage to spinal progensitor cells to promote regenerative neurogenesis in zebrafish.