T
Thibaut Crépin
Researcher at University of Grenoble
Publications - 50
Citations - 3835
Thibaut Crépin is an academic researcher from University of Grenoble. The author has contributed to research in topics: RNA & Polymerase. The author has an hindex of 22, co-authored 45 publications receiving 3478 citations. Previous affiliations of Thibaut Crépin include Centre national de la recherche scientifique & Commissariat à l'énergie atomique et aux énergies alternatives.
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Journal ArticleDOI
The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit
Alexandre Dias,Denis Bouvier,Thibaut Crépin,Andrew A. McCarthy,Darren J. Hart,Florence Baudin,Stephen Cusack,Rob W.H. Ruigrok +7 more
TL;DR: In this paper, the amino-terminal 209 residues of the PA subunit contain the active site of the endonuclease active site, which is shown to be strongly activated by manganese ions, matching observations reported for the intact trimeric polymerase.
Journal ArticleDOI
An Antifungal Agent Inhibits an Aminoacyl-tRNA Synthetase by Trapping tRNA in the Editing Site.
Fernando Rock,Weimin Mao,A. D. Yaremchuk,M. A. Tukalo,Thibaut Crépin,Huchen Zhou,Yong Kang Zhang,Vincent Hernandez,Tsutomu Akama,Stephen J. Baker,Jacob J. Plattner,Lucy Shapiro,Susan A. Martinis,Stephen J. Benkovic,Stephen Cusack,M. R. K. Alley +15 more
TL;DR: The broad-spectrum antifungal 5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole (AN2690), in development for the treatment of onychomycosis, inhibits yeast cytoplasmic leucyl-tRNA synthetase by formation of a stable tRNALeu-AN 2690 adduct in the editing site of the enzyme.
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The Structural Basis for CAP Binding by Influenza Virus Polymerase Subunit Pb2.
Delphine Guilligay,Franck Tarendeau,Patricia Resa-Infante,Rocío Coloma,Thibaut Crépin,Peter Sehr,Joe Lewis,Rob W.H. Ruigrok,Juan Ortín,Darren J. Hart,Stephen Cusack +10 more
TL;DR: Binding and functional studies with point mutants confirm that the identified site is essential for cap binding in vitro and cap-dependent transcription in vivo by the trimeric polymerase complex, and will allow efficient structure-based design of new anti-influenza compounds inhibiting viral transcription.
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Structural insight into cap-snatching and RNA synthesis by influenza polymerase
Stefan Reich,Delphine Guilligay,Alexander Pflug,Hélène Malet,Imre Berger,Thibaut Crépin,Darren J. Hart,Thomas Lunardi,Max H. Nanao,Rob W.H. Ruigrok,Stephen Cusack +10 more
TL;DR: Crystal structures of bat influenza A and human influenza B polymerases (FluA and FluB), bound to the viral RNA promoter, are used to give mechanistic insight into these distinct processes.
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Nucleoproteins and nucleocapsids of negative-strand RNA viruses.
TL;DR: A hallmark of negative-strand RNA viruses is that their genomes never exist as free RNA, but instead are always assembled with many copies of a single nucleoprotein (N) to form highly stable nucleocapsids.