Todd R. Klaenhammer

TL;DR: It is suggested that the Hsp+ phenotype may result from plasmid-induced abortive infection of phage dependent on the presence of pTR2030, and determinants for conjugal transfer ability (Tra+) are located on p TR2030.

TL;DR: The lactobacilli occupy a diverse group of specialized habitats that include fermenting food and the oral, vaginal, and gastrointestinal tracts of humans and animals and are said to have beneficial effects in the gastrointestinal tract.

TL;DR: A upp-based counterselective chromosomal integration system was established and employed for targeting the lipoteichoic acid (LTA) synthesis gene, ltaS, in L. gasseri ATCC 33323, and an integration knockout mutant was generated with insertion inactivation at the lTAS locus.

TL;DR: In this paper, a phage defense rotation strategy for use in successive fermentations of a substrate in a fermentation plant is disclosed, where the strategy comprises (a) fermenting substrate with a first bacterial culture comprising a bacterial strain capable of fermenting the substrate and, preferably, carrying a first phage defence mechanism; and then (b) fermentation the substrate with another bacterial culture, comprising a second bacterial strain isogenic with the first bacterial strain, wherein the second strain carries a PHV defense mechanism different from the PHV defence mechanism carried by the first strain.

TL;DR: Results elucidate the impact of ltaS deletion on the transcriptome and extracellular proteins of L. acidophilus, suggesting roles of LTA in cell morphology and ion homeostasis as a structural component of the Gram positive cell wall.