T
Toshihiro Yasuda
Researcher at University of Fukui
Publications - 230
Citations - 4121
Toshihiro Yasuda is an academic researcher from University of Fukui. The author has contributed to research in topics: Deoxyribonuclease I & Single-nucleotide polymorphism. The author has an hindex of 31, co-authored 230 publications receiving 3890 citations. Previous affiliations of Toshihiro Yasuda include Shimane University & City of Hope National Medical Center.
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Journal ArticleDOI
Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method
TL;DR: The SRED can be used for quantitation of deoxyribonuclease I with a limit of detection of 2 x 10(-6) unit, and the activity of 17 different human tissues and body fluids from healthy donors was measured.
Journal Article
Human serum deoxyribonuclease I (DNase I) polymorphism: pattern similarities among isozymes from serum, urine, kidney, liver, and pancreas.
TL;DR: A combination technique of polyacrylamide-gel isoelectric-focusing electrophoresis and the newly developed zymogram method have led to the discovery of genetic polymorphism of human serum DNase I.
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Quantitative change in mitochondrial DNA content in various mouse tissues during aging.
TL;DR: The Western blot analysis of mtDNA-encoded cytochrome c oxidase subunit III (MTCO3) demonstrated that the expression levels of this protein in the heart and skeletal muscle increase with age in parallel with the mtDNA content, confirming that the mt DNA content of tissues changes during aging.
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Molecular Cloning of the cDNA Encoding Human Deoxyribonuclease II
Toshihiro Yasuda,Haruo Takeshita,Reiko Iida,Tamiko Nakajima,Osamu Hosomi,Yoshimitsu Nakashima,Koichiro Kishi +6 more
TL;DR: A rapid amplification of cDNA ends method, using degenerate oligonucleotides based upon the N-terminal amino acid sequence of human hepatic deoxyribonuclease II (DNase II), allowed a novel cDNA encoding DNase II to be constructed from thyroid gland RNA.
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Human urine deoxyribonuclease II (DNase II) isoenzymes: a novel immunoaffinity purification, biochemical multiplicity, genetic heterogeneity and broad distribution among tissues and body fluids.
TL;DR: Genetic studies of urinary enzyme activity and comparative studies on the activity in urine, semen and leukocytes from the same individuals suggest that the enzyme activity level of DNase II may be under genetic control.