T
Toyoaki Natsume
Researcher at National Institute of Genetics
Publications - 37
Citations - 1709
Toyoaki Natsume is an academic researcher from National Institute of Genetics. The author has contributed to research in topics: DNA replication & Kinetochore. The author has an hindex of 18, co-authored 34 publications receiving 1188 citations. Previous affiliations of Toyoaki Natsume include Graduate University for Advanced Studies & University of Dundee.
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Journal ArticleDOI
Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors
Toyoaki Natsume,Tomomi Kiyomitsu,Tomomi Kiyomitsu,Yumiko Saga,Yumiko Saga,Masato T. Kanemaki,Masato T. Kanemaki +6 more
TL;DR: This work has developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins in human HCT116 and mouse embryonic stem (ES) cells by using donor constructs that harbor synthetic short homology arms.
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Kinetochores Coordinate Pericentromeric Cohesion and Early DNA Replication by Cdc7-Dbf4 Kinase Recruitment
Toyoaki Natsume,Carolin A. Müller,Yuki Katou,Renata Retkute,Marek Gierlinski,Hiroyuki Araki,J. Julian Blow,Katsuhiko Shirahige,Conrad A. Nieduszynski,Tomoyuki Tanaka +9 more
TL;DR: This work shows that the budding yeast Dbf4-dependent kinase (DDK) accumulates at kinetochores in telophase, facilitated by the Ctf19 kinetichore complex, and finds the central mechanism by which kinetchores orchestrate early S phase DNA replication and robust sister chromatid cohesion at microtubule attachment sites.
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Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble
TL;DR: It is demonstrated that induced cortical targeting of NuMA, but not dynein, is sufficient for spindle pulling, and proposed that cortical Dynein-Dynactin-NuMA (DDN) clusters act as the core force-generating machinery that organizes a multi-arm ensemble reminiscent of the kinetochore.
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Xrn2 accelerates termination by RNA polymerase II, which is underpinned by CPSF73 activity.
Joshua D. Eaton,Lee Davidson,Lee Davidson,David L.V. Bauer,Toyoaki Natsume,Toyoaki Natsume,Masato T. Kanemaki,Masato T. Kanemaki,Steven West +8 more
TL;DR: Efficient termination on most protein-coding genes involves CPSF73-mediated RNA cleavage and cotranscriptional degradation of polymerase-associated RNA by Xrn2, however, as CPSF 73 loss caused more extensive readthrough transcription than Xrn1 elimination, it likely plays a more underpinning role in termination.
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Conditional Degrons for Controlling Protein Expression at the Protein Level
TL;DR: Direct protein depletion (or protein knockdown approaches) is advantageous in terms of specificity, reversibility, and time required for depletion, which can be achieved by fusing a POI with a protein domain called a degron that induces rapid proteolysis of the fusion protein.