Vincent van Hoef

TL;DR: Findings highlight the importance of mTOR-regulated metabolic processes for immune cell functions and argue for implementation of IL-15 in adoptive NK-cell cancer therapy.

TL;DR: This chronic ISR program is characterized by persistently elevated uORF mRNA translation and concurrent gene expression reprogramming, which permits simultaneous stress sensing and proteostasis and establishes a molecular basis for understanding human diseases associated with ER dysfunction.

TL;DR: A non-linear sucrose gradient is optimized which reproducibly enriches for efficiently translated mRNA in only one or two fractions, thereby reducing sample handling 5–10-fold and presenting a polysome-profiling technique applicable to large study designs, primary cells and frozen tissue samples such as those collected in biobanks.

TL;DR: Polysome profiling developed for small samples shows that, during tumor growth, gene expression in TAMs is predominately modulated via mRNA-selective changes in translational efficiencies, and selective changes of mRNA translation depending on MNK2 signaling represents a key node regulating macrophage antiinflammatory functions.

TL;DR: A non-linear sucrose gradient is optimized which reproducibly enriches for mRNAs associated with >3 ribosomes in only one or two fractions, thereby reducing sample handling 5-10 fold and identifying a translatome associated with p53 status under serum starvation.