Vladimir S. Prassolov

TL;DR: Current ideas on mechanisms used by 40S ribosomal subunits to cope with complex 5′UTRs are modified and called into question the conception that every long GC-rich5′UTR working with a high efficiency has to contain an IRES.

TL;DR: It is shown that the 216-nt long 5′-UTR of Hsp70 mRNA acts as an IRES that directs ribosomes to the downstream start codon by a cap-independent mechanism, and that the IRES activity requires integrity of almost the entire sequence of the 5′ -UTR.

TL;DR: Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of human carcinoma cells inhibited the growth of the human tumour xenografts.

TL;DR: It is not mandatory to invoke the IRES hypothesis, at least for some mRNAs, to explain their preferential translation when eIF4E is partially inactivated, because in cultured cells or cytoplasmic extracts both the level of stimulation with the cap and the overall translation activity do not correlate with the cumulative energy of the secondary structure of the tested 5′ UTRs.

TL;DR: The gene expression database of normal human tissues based on uniformly screened original sequencing data was constructed and deposited, finding that overall 463 biosamples showed tissue-specific rather than platform- or database-specific clustering and could be aggregated in a single database termed Oncobox Atlas of Normal Tissue Expression (ANTE).