Walter W. Chen

TL;DR: Results reveal that certain breast cancers are dependent upon increased serine pathway flux caused by PHGDH overexpression and demonstrate the utility of in vivo negative-selection RNAi screens for finding potential anticancer targets.

TL;DR: A CRISPR-based genetic screen was used to identify genes whose loss sensitizes human cells to phenformin, a complex I inhibitor, and yielded GOT1, the cytosolic aspartate aminotransferase, loss of which kills cells upon ETC inhibition.

TL;DR: In this article, the authors identify GATOR as a complex that interacts with the Rags and is composed of two sub-complexes called GATOR1 and -2, and reveal that, like other mTORC1 regulators, Rag function can be deregulated in cancer.

TL;DR: In this article, the authors used a CRISPR-based genetic screen to identify genes whose loss sensitizes human cells to phenformin, a complex I inhibitor, and yielded GOT1, the cytosolic aspartate aminotransferase, loss of which kills cells upon ETC inhibition.

TL;DR: In this article, a continuous-flow culture apparatus is used to maintain proliferating cancer cells in low-glucose conditions, demonstrating that mitochondrial oxidative phosphorylation (OXPHOS) is essential for optimal proliferation in these conditions; the most sensitive cell lines are defective in OXPHOS upregulation and may therefore be sensitive to current antidiabetic drugs that inhibit OPHOS.