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Xiaowei Xie
Researcher at Sun Yat-sen University
Publications - 10
Citations - 1336
Xiaowei Xie is an academic researcher from Sun Yat-sen University. The author has contributed to research in topics: CRISPR & Gene. The author has an hindex of 8, co-authored 9 publications receiving 1164 citations. Previous affiliations of Xiaowei Xie include Peking Union Medical College.
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Journal ArticleDOI
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
Puping Liang,Yanwen Xu,Xiya Zhang,Chenhui Ding,Rui Huang,Zhen Zhang,Jie Lv,Xiaowei Xie,Yuxi Chen,Yujing Li,Sun Ying,Yaofu Bai,Zhou Songyang,Wenbin Ma,Canquan Zhou,Junjiu Huang +15 more
TL;DR: It is found that CRISPR/Cas9 could effectively cleave the endogenous β-globin gene (HBB), however, the efficiency of homologous recombination directed repair (HDR) of HBB was low and the edited embryos were mosaic.
Journal ArticleDOI
Correction of β-thalassemia mutant by base editor in human embryos
Puping Liang,Chenhui Ding,Hongwei Sun,Xiaowei Xie,Yanwen Xu,Xiya Zhang,Ying Sun,Yuanyan Xiong,Wenbin Ma,Yongxiang Liu,Yali Wang,Jianpei Fang,Dan Liu,Zhou Songyang,Zhou Songyang,Canquan Zhou,Junjiu Huang +16 more
TL;DR: The feasibility of curing genetic disease in human somatic cells and embryos by base editor system is demonstrated and base editor variants, with narrowed deamination window, could promote G-to-A conversion at HBB −28 site precisely in human embryos.
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Genome-wide profiling of adenine base editor specificity by EndoV-seq
Puping Liang,Xiaowei Xie,Xiaowei Xie,Shengyao Zhi,Hongwei Sun,Xiya Zhang,Yu Chen,Yuxi Chen,Yuanyan Xiong,Wenbin Ma,Dan Liu,Junjiu Huang,Zhou Songyang +12 more
TL;DR: This study presents the first detection method to evaluate genome-wide off-target effects of ABE, and reveals possible similarities and differences between ABE and canonical Cas9 nuclease.
Journal ArticleDOI
Effective gene editing by high-fidelity base editor 2 in mouse zygotes
Puping Liang,Hongwei Sun,Ying Sun,Xiya Zhang,Xiaowei Xie,Jinran Zhang,Zhen Zhang,Yuxi Chen,Chenhui Ding,Yuanyan Xiong,Wenbin Ma,Dan Liu,Junjiu Huang,Zhou Songyang +13 more
TL;DR: The feasibility of generating point mutations in mouse by base editing is demonstrated, and the need to carefully optimize base editing systems in order to eliminate proximal-site deamination is underscored.
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Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9.
Xiya Zhang,Puping Liang,Chenhui Ding,Zhen Zhang,Jianwen Zhou,Xiaowei Xie,Rui Huang,Ying Sun,Hongwei Sun,Jinran Zhang,Yanwen Xu,Zhou Songyang,Junjiu Huang +12 more
TL;DR: SaCas9 can specifically cleave the target gene locus, leading to successful gene knock-out and precise knock-in in mouse zygotes, and highlight the potential of using SaCas9 for genome editing in preimplantation embryos and producing gene-modified animal models.