Yangyang Shao

TL;DR: A functional single-chromosome yeast from a Saccharomyces cerevisiae haploid cell containing sixteen linear chromosomes is created by successive end-to-end chromosome fusions and centromere deletions to demonstrate an approach to exploration of eukaryote evolution with respect to chromosome structure and function.

TL;DR: Both SY14 and SY15 cells showed no detectable changes in the restriction enzyme digestion pattern of their genomes compared with their descendant cells at passage 100, suggesting that the single chromosome yeasts are able to maintain stable genomes.

TL;DR: This work described a new method designated as "MEGA (Multiple Essential Genes Assembling) deletion and replacement" that functions by assembling multiple essential genes in an E. coli-S.

TL;DR: It is found that the deletion of CDC13 leads to telomere erosion and intrachromosome end-to-end fusion, which depends on Rad52 but not Yku, verifying the default role of intact telomeres in inhibiting chromosome fusion.

TL;DR: This protocol provides experimentally derived guidelines for the generation of functional chromosome fusions in yeast, especially for the deletion of repetitive sequences, the determination of the fusion order and cleavage sites, and primary evaluation of the functionality of chromosomes fusions.