Zhenfang Wu

TL;DR: A functional single-chromosome yeast from a Saccharomyces cerevisiae haploid cell containing sixteen linear chromosomes is created by successive end-to-end chromosome fusions and centromere deletions to demonstrate an approach to exploration of eukaryote evolution with respect to chromosome structure and function.

TL;DR: The complete genome sequence of H. hispanica strain CGMCC 1.2049, consisting of two chromosomes and one megaplasmid is reported, which is significant for studying archaeal genetics, metabolism, and virus-host interactions.

TL;DR: A comparison of the origin-associated Orc/Cdc6 homologs and the corresponding predicted ORB elements revealed that the replication origins in a given haloarchaeon are quite diverse, while different halo archaea can share a few conserved origins.

TL;DR: This review summarizes the research progress in understanding of archaeal replication origins with particular focus on the utilization, control and evolution of multiple replication origins in haloarchaea.

TL;DR: The active replication origins in the three replicons of a halophilic archaeon, Haloarcula hispanica, are investigated by extensive gene deletion, DNA mutation and genome-wide marker frequency analyses, revealing that individual origins are specifically dependent on their co-located cdc6 genes and a single active origin/cdc6 pairing is essential and sufficient for each replicon.