You Li

TL;DR: The results indicate that structure‐based computational design can be successfully applied to further improve the binding of high‐affinity antibodies and improve the single‐mutant success rate.

TL;DR: The anti-tumor properties of BIIB036 validate Fn14 as a promising target in oncology and demonstrate its potential therapeutic utility in multiple solid tumor indications.

TL;DR: In this paper, the authors proposed a method of humanizing antibodies in which selected CDR residues, and optionally adjacent FR residues, are changed in order to accommodate differences in FR amino acid sequences between donor and acceptor antibodies.

TL;DR: Application of both approaches to the humanization of anti-α4 integrin antibody HP1/2 is presented and the concept of the hybrid humanization approach that retains "difficult to match" murine framework amino acids and uses de novo CDR design to minimize murine amino acid content and reduce cell-mediated cytotoxicity liabilities is discussed.

TL;DR: A design approach was taken to investigate the feasibility of replacing single complementarity determining region (CDR) antibody loops with designs that took the qualitatively desired conformation, confirming that loop replacement by design is feasible.