Yuri Sokolov

TL;DR: It is reported that soluble oligomers from several types of amyloidogenic proteins and peptides specifically increase lipid bilayer conductance regardless of the sequence, while fibrils and soluble low molecular weight species have no effect.

TL;DR: The preparation of relatively homogeneous preparations of APFs and an antiserum selective for APFs (αAPF) compared with prefibrillar oligomers (PFOs) and fibrils is reported, suggesting that lipid vesicles catalyze the conversion of PFOs into APFs.

TL;DR: This paper addresses the mechanism of the amyloid hypothesis of Alzheimer's toxicity, and finds that only oligomers increase the conductance of lipid bilayers and patch-clamped mammalian cells, producing almost identical current–voltage curves in both preparations.

TL;DR: The interaction of soluble prefibrillar amyloid beta (Abeta(1-42)-oligomers with bilayer models is examined, observing also dramatic increases in ion current at micromolar peptide concentrations and the Abeta-induced membrane conductance shows a nonlinear dependence on the peptide concentration in the membrane.

TL;DR: The voltage-gated K+ channel of T-lymphocytes, Kv1.3, was heterologously expressed in African Green Monkey kidney cells (CV-1) using a vaccinia virus/T7 hybrid expression system and was purified to near-homogeneity by a single nickel-chelate chromatography step.