Showing papers by "Central Tuber Crops Research Institute published in 2022"

Novel Levilactobacillus brevis-based formulation for controlling cell proliferation, cell migration and gut dysbiosis

Abstract: The objective of the study was to formulate a novel Levilactobacillus brevis enriched nutraceutical and to study its functional property in vitro in cancer cell lines and in vivo in Salmonella enterica serovar Typhimurium infected mouse model. The formulation was prepared through the fermentation of carrot and beetroot extracts using L. brevis MTCC 4460 and optimized by response surface methodology and artificial neural networking. The optimized formulation (3.23 mg/ml lactic acid) could be obtained through 48 h fermentation with 2% of bacterial inoculum, 0.67% additional sugar and 30.11% of beetroot extract. The L. brevis MTCC 4460 content in the optimized product was 4 × 109 CFU/ml. GC-MS study indicated the generation of some novel flavouring and bioactive compounds such as γ-decalactone, and 1,2:5,6-dianhydrogalactitol during the fermentation. In vitro study with HCT116 and MDA-MB-231 cancer cell lines elucidated better antiproliferative and antimigratory effect of the optimized formulation. In vivo studies showed that the L. brevis MTCC 4460 could colonize in the colon of the mouse fed with the optimized product. In addition, the formulation effectively prevented Salmonella-induced colitis in the mouse model. Based on the aforesaid findings, the optimized formulation can be recommended as a potential dietary supplement for a healthy lifestyle.

Root system architectural and growth responses of crop plants to mineral nutrition under moisture stress and its implications in drought tolerance

Abstract: The world agriculture is facing to meet the challenges of growing food demands under climate change scenario with limited natural resources, and water is the primary factor limiting the crop productivity in different parts of the world. Much of the research attention has been focused on above-ground plant parts responses with an aim to improve drought tolerance and very less attention has been focused on roots and their ability to sustain crop productivity under water limiting environment, despite root, being a sensing organ for nutrient and moisture. In this chapter, we discussed the role of nutrients on root architecture and growth with an aim to explore the crop root system architecture (RSA) optimization by nutrient management and thus increase drought tolerance. In this chapter, we demonstrate the importance of nutrient management for achieving favorable RSA and root growth responses in different crop plants and its implications in drought tolerance. This will open new implementable agronomic management strategies for drought tolerance. Moreover, a deeper understanding of molecular sensing and regulation of RSA by nutrients under water limiting condition could support the development of climate resilient varieties by tailoring the specific root traits for efficient absorption of limiting nutrient and water, simultaneously.

Electrospinning of Biofibers and their Applications

Abstract: There is lot of uncertainty attached to petroleum supply and the entire world is searching for alternatives. Natural resources are explored in this context and materials derived from bioresources are being produced. For this reason, materials derived from natural resources have become increasingly popular over the last two decades. This is also highlighted as non-renewable resources are getting depleted very rapidly. Researchers from academia and industry are very keen to develop valuable products from natural resources. Bio materials are gaining importance and technologies are emerging to handle these biomaterials which are more sensitive than conventional technologies. This chapter narrates the recent development of biomaterials, biofibers, biopolymers and biocomposites and the electrospinning process most widely used in biomaterials. The performances of biomaterials and biocomposites are reviewed because they have a wide range of applications and good future prospects in new generation materials and processes.