Korean Ocean Research and Development Institute

About: Korean Ocean Research and Development Institute is a facility organization based out in Busan, South Korea. It is known for research contribution in the topics: Sea surface temperature & Gene. The organization has 1770 authors who have published 3032 publications receiving 50142 citations.

Seasonal distribution of planktic foraminifers in the east sea (sea of japan), a large marginal sea of the northwest pacific

Abstract: A time series sediment trap was deployed in the interior of the East Sea (Sea of Japan) (39°40′N, 132°24′E) for the period from July 1994 to July 1995. Total foraminifera flux varied from 3 to 8,200 individuals/m 2 /day, with major peaks during late fall (November) and winter (December and January), and a minor peak during spring (March–May). Foraminiferal assemblages were dominated by both right- and left-coiled Neogloboquadrina pachyderma , N. dutertrei , and Globigerina bulloides , and their fluxes varied seasonally. The highest flux of both right- and left-coiled N. pachyderma throughout all seasons indicates that the study site was dominated by the cold Subarctic water mass. The influx from the cold North Korean Current was stronger than that of the warm Tsushima Current in the spring. This cold water influx is evidenced by the highest flux of left coiled N. pachyderma and Globigerina bulloides , and the absence of N. dutertrei . The dominance of right-coiled N. pachyderma during January, July, and November–December suggests a greater influence of the warm Tsushima Current. The absence of N. dutertrei at the study site and the presence of N. dutertrei in the North Pacific in spring suggests that the study site in the Japan Basin is isolated from the North Pacific Ocean during the spring. G. bulloides and N. dutertrei dominate during March and November, respectively. The onset of the winter monsoon (late October–November) is responsible for the planktic foraminifera flux peak during November.

Critical Factors to High Thermostability of an ${\alpha}$-Amylase from Hyperthermophilic Archaeon Thermococcus onnurineus NA1

Abstract: Genomic analysis of a hyperthermophilic archaeon, Thermococcus onnurineus NA1 [1], revealed the presence of an open reading frame consisting of 1,377 bp similar to alpha-amylases from Thermococcales, encoding a 458-residue polypeptide containing a putative 25-residue signal peptide. The mature form of the alpha-amylase was cloned and the recombinant enzyme was characterized. The optimum activity of the enzyme occurred at 80 degrees C and pH 5.5. The enzyme showed a liquefying activity, hydrolyzing maltooligosaccharides, amylopectin, and starch to produce mainly maltose (G2) to maltoheptaose (G7), but not pullulan and cyclodextrin. Surprisingly, the enzyme was not highly thermostable, with half-life (t(1/2)) values of 10 min at 90 degrees C, despite the high similarity to alpha-amylases from Pyrococcus. Factors affecting the thermostability were considered to enhance the thermostability. The presence of Ca2+ seemed to be critical, significantly changing t(1/2) at 90 degrees C to 153 min by the addition of 0.5 mM Ca2+. On the other hand, the thermostability was not enhanced by the addition of Zn2+ or other divalent metals, irrespective of the concentration. The mutagenetic study showed that the recovery of zinc-binding residues (His175 and Cys189) enhanced the thermostability, indicating that the residues involved in metal binding is very critical for the thermostability.

Identification of bioactive metabolites against adenosine A1 receptor using NMR-based metabolomics

Abstract: Marine sponges are relatively less explored for their chemical features but highly anticipated resource for bioactive compounds. In this paper we report the screening of marine sponges crude extracts for their potential to bind the adenosine A1 receptor. Many samples showed very promising activity and in order to identify the active components, a metabolomics-chemometrics approach is employed. Nuclear magnetic resonance spectroscopy is used for the metabolic profiling of the marine sponges and partial least squares (PLS) and orthogonal PLS (OPLS) algorithms are used to correlate the metabolomics with bioactivity data. Using several two dimensional-NMR techniques, the resonances responsible for the separation of high activity samples from the medium and low activity samples were identified as associated to metabolites like halisulfate 1, halisulfate 3–5, and suvanine (1–5), all belongs to sesterterpenes class. The reference compounds for these metabolites are also tested for the activity, which endorse the findings of the applied methodology.

Structural Evidence for Antihypertensive Effect of an Antioxidant Peptide Purified from the Edible Marine Animal Styela clava.

Abstract: This study investigated the antihypertensive effects of an antioxidant peptide, Leu-Trp-His-Thr-His (LWHTH), purified from Styela clava peptic hydrolysate, to assess the bioactivity of the peptide ...