Mangalayatan University

About: Mangalayatan University is a education organization based out in Aligarh, Uttar Pradesh, India. It is known for research contribution in the topics: Epitope & Population. The organization has 99 authors who have published 106 publications receiving 1697 citations.

Medicinal plants with neuropharmacological properties from indian origin

Abstract: Neuropharmacology is the scientific study of the effects of drugs on the central nervous system. Its primary focus is actions of medications for psychiatric and neurologic disorders as well as those of drugs of abuse. The goal of Neuropharmacology is to apply information about drugs and their mechanisms of action, develop safer and more effective treatments and eventually curative and preventive measures for a host of nervous system abnormalities. There are a number of drugs being used in the traditional medicine for treatment of various CNS disorders and presently many of these drugs are being explored scientifically to ascertain their CNS activities. Significant number of studies has been performed to find alternative treatment for diseases of the nervous forum by identifying structures with activity at the central nervous system. However, most of the screenings are usually conducted on an ad hoc basis and not systematically. The initial purpose of this review is to summarize plants with neuropharmacological activities, in particular, those that have not been fully studied and that have molecular mechanisms whose active constituents are responsible for the activities remained to be identified. The second purpose was to identify potential target plants for future studies of new and alternative therapies for the treatment of neurological disorders and neurodegenerative diseases.

A comparative study of inclusion complexes of flunarizine with alpha (α-CD) and beta-cyclodextrin (β-CD)

Abstract: A detailed NMR (1H, COSY, and ROESY) spectroscopic study of complexation of Flunarazine (FL) with α- and β-CD was carried out. 1H NMR titration studies confirmed the formation of FL/α-CD and FL/β-CD complexes as evidenced by chemical shift variations of the proton resonances of both the CDs and FL. The stoichiometry of the complexes was determined to be 1:2 (FL/α-CD) and 1:1 (FL/β-CD) and overall binding constants were also calculated. It was confirmed with the help of ROESY spectral data that only one of the F-substituted aromatic ring and phenyl ring penetrate the α-CD cavity while both F-substituted aromatic rings as well as phenyl ring penetrates the β-CD cavity during complexation. The binding modes of FL/CD cavity interactions derived from ROESY experimental data show that the resulting complex of FL with β-CD possesses better induced fit interaction as compared to α-CD, which is responsible for the enhanced molecular stability with β-CD in comparison to α-CD. The mode of penetration of guest into the CD cavity and structures of the complexes has been established.

Sequence and Structure Based Binding Prediction Study of HLA Class I and cTAP Binding Peptides for Japanese Encephalitis Vaccine Development

Abstract: Japanese encephalitis is a major threat in developing countries, even the availability of several conventional vaccines, which demand development of more effective vaccines. The present study used propred I and Immune Epitope Database Artificial Neural Network (ANN) algorithm (IEDB-ANN) to identify the conserve and promiscuous T cell epitopes from JEV proteome followed by structure based analysis of potential epitopes. Among all identified 102 epitopes, ten epitope were promiscuous but two epitopes of glycoprotein viz. 55LVTVNPFVA63 and 38IPIVSVASL46 were found most promiscuous, highly conserved and high population coverage in comparison of known antigenic positive control peptides. The B cell epitopes of glycoprotein also share these two T cell epitopes revealed by BCPred algorithm which can be a basis to confer the protection by neutralizing antibody combined with an effective cell-mediated response. Further, Autodock 4.2 and NAMD–VMD molecular dynamics simulation were used for docking and molecular dynamics simulation respectively, to validate epitope and allele complex binding stability. The 3D structure models were generated for epitopes and corresponding HLA allele by Pepstr and Modeller 9.10 respectively. Epitope LVTVNPFVA–B5101 allele complex showed best energy minimization and stability over the time window during simulation. Here we also present the binding sequel of epitope LVTVNPFVA and its eventual transport through cTAP1 (PDB ID: 1JJ7) revealed by Autodock 4.2, which is an essential path for HLA class I binding epitopes to elicit immune response. The docking experiment of epitope LVTVNPFVA and cTAP1 very well show a 2 H-bond with a binding energy of −1.88 kcal/mol and other binding state of epitope forming no H-bond with a binding energy of −1.13 kcal/mol in the lower area of cTAP1 cavity. These results show a smooth pass through of the epitope across the channel of cTAP1. Overall, identified peptides have potential application in the design and development of short peptide based vaccines and diagnostic agents for Japanese encephalitis.

Antimicrobial activity of barleria prionitis on pathogenic strains

Abstract: Objectives: The present study was planned to investigate the antimicrobial activity of different part of the plant drug . Therefore, the preliminary successive solvent extraction and chemical test revealed the presence of secondary metabolites in various extracts, and provide us clue for further investigation . Methods: Different solvents i.e., Chloroform, Acetonitrile, Ethanol, was used for extraction of active secondary metabolites by the Soxhlet method. Percentage of yield 4 , and antimicrobial activity by gradient plate technique [13]. Statistical analysis has done by standard error by mean (SEM), in which n=3. Results: The significant antimicrobial activity of leaf, stem and root along with 1.2±0.0136 mg/ml to 4±0.0128 mg/ml on bacterial strain and 9.4±0.0128 mg/ml to 14.5±0.0149 mg/ml MIC value with SEM. The highest percentage of yield was observed in Ethanol, chloroform and Acetonitrile i. e from 3.7% to 5.36%. Various numbers of secondary metabolites were extracted from 1 to 4 in Barleria prionitis Linn. Conclusion: Ethanol has been shown as a best solvent to yield, extracted components and antimicrobial effects prospects while acetonitrile and chloroform were also good solvents to extract secondary metabolite components. Keywords: Barleria prionitis , Secondary metabolites, Antimicrobial activity, Solvents.