Mangalayatan University

About: Mangalayatan University is a education organization based out in Aligarh, Uttar Pradesh, India. It is known for research contribution in the topics: Epitope & Population. The organization has 99 authors who have published 106 publications receiving 1697 citations.

Antipsychotic activity of aqueous ethanolic extract of tinospora cordifolia in amphetamine challenged mice model.

Abstract: Tinospora cordifolia is reported to have CNS active principle and is used for the treatment of various neurological disorders. Hence, the effect of aqueous ethanolic extract of Tinospora cordifolia was investigated for its putative antipsychotic activity using amphetamine challenged mice model. Haloperidol (1 mg/kg i.p.) was administered acutely to mice as standard drug. Control animals received vehicle (10% DMSO). The in vivo receptor binding studies were carried out to correlate the antipsychotic activity of the extract with its capacity to bind to the DAD2 receptor. The results in SLA showed that the hydro alcoholic extract of the stems of Tinospora cordifolia at a dose level of 250 mg/kg and 500 mg/kg showed no significant antipsychotic activity in amphetamine induced hyperactivity in mice when compared to standard. Extract alone treated group at a dos level of 250 mg/kg and 500 mg/kg showed a decreased in locomotor activity when compared to the control. The plant extract increased the DAD(2) receptor binding in a dose dependent manner in treated mice compared to the control group.

Antioxidant and Antimicrobial Activity of Nutmeg ( Myristica fragrans )

Abstract: Publisher Summary This chapter highlights the antioxidant and antimicrobial activity of nutmeg. Volatile essential oil and various extracts of nutmeg seeds have been reported to have many pharmacological properties, including antioxidant, antimicrobial, insecticidal, anti-amebic, and anticarcinogenic activity. The antioxidant capacity of nutmeg seeds can be measured by various chemical assays, such as estimation of total phenolic concentration, capacity to scavenge the stable free radical DPPH (2,2-diphenyl-1-picrylhydrazyl), ferric reducing/antioxidant power assay (FRAP), inhibition of lipid peroxidation, and inhibition of bleaching of β-carotene. Antioxidant properties are contributed by the variety of active phytochemicals, including vitamins, carotenoids, terpenoids, alkaloids, flavonoids, lignans, simple phenols, and phenolic acids. Various extracts and the essential oil of nutmeg seeds have presented strong antimicrobial activity against gram-positive and gram-negative bacteria, as well as a variety of fungi. Due to its high antioxidant and antimicrobial activities, nutmeg could be considered as a significant natural source of antioxidants and antimicrobials. Nutmeg, being a natural product, can offer more safety to people and the environment and is considered to be less of a risk for resistance development by pathogenic microorganisms.

Screening and structure-based modeling of T-cell epitopes of Nipah virus proteome: an immunoinformatic approach for designing peptide-based vaccine

Abstract: Identification of Nipah virus (NiV) T-cell-specific antigen is urgently needed for appropriate diagnostic and vaccination. In the present study, prediction and modeling of T-cell epitopes of Nipah virus antigenic proteins nucleocapsid, phosphoprotein, matrix, fusion, glycoprotein, L protein, W protein, V protein and C protein followed by the binding simulation studies of predicted highest binding scorers with their corresponding MHC class I alleles were done. Immunoinformatic tool ProPred1 was used to predict the promiscuous MHC class I epitopes of viral antigenic proteins. The molecular modelings of the epitopes were done by PEPstr server. And alleles structure were predicted by MODELLER 9.10. Molecular dynamics (MD) simulation studies were performed through the NAMD graphical user interface embedded in visual molecular dynamics. Epitopes VPATNSPEL, NPTAVPFTL and LLFVFGPNL of Nucleocapsid, V protein and Fusion protein have considerable binding energy and score with HLA-B7, HLA-B*2705 and HLA-A2MHC class I allele, respectively. These three predicted peptides are highly potential to induce T-cell-mediated immune response and are expected to be useful in designing epitope-based vaccines against Nipah virus after further testing by wet laboratory studies.