Università Campus Bio-Medico

About: Università Campus Bio-Medico is a education organization based out in Rome, Italy. It is known for research contribution in the topics: Population & Medicine. The organization has 2829 authors who have published 8519 publications receiving 193689 citations. The organization is also known as: Universita Campus Bio-Medico & Campus Bio-Medico University.

Early experiences in mitotic cells recognition on HEp-2 slides

Abstract: Indirect immunofluorescence (IIF) imaging is the recommended laboratory technique to detect autoantibodies in patient serum, but it suffers from several issues limiting its reliability and reproducibility. IIF slides are observed by specialists at the fluorescence microscope, reporting fluorescence intensity and staining pattern and looking for mi-totic cells. Indeed, the presence of such cells is a key factor to assess the correctness of slide preparation process and the reported staining pattern. Therefore, the ability to detect mitotic cells is needed to develop a complete computer-aided-diagnosis system in IIF, which can support the specialists from image acquisition up to image classification. Although recent research in IIF has been directed to image acquisition, image segmentation, fluorescence intensity classification and staining pattern recognition, no works presented methods suited to classify such cells. Hence, this paper presents an heterogeneous set of features used to describe the peculiarities of mitotic cells and then tests five classifiers, belonging to different classification paradigms. The approach has been evaluated on an annotated dataset of mitotic cells. The measured performances are promising, achieving a classification accuracy of 86.5 %.

Anteromedial Portal Versus Transtibial Drilling Techniques in Anterior Cruciate Ligament Reconstruction: Any Clinical Relevance? A Retrospective Comparative Study

Abstract: Purpose This study aimed to undertake a retrospective analysis of prospectively collected data comparing, at a minimum follow-up of 5 years (78.1 ± 5.3 months v 75.6 ± 4.8 months), the clinical, functional, and radiographic outcomes of 2 homogeneous groups of athletes who had undergone arthroscopic single-bundle autologous hamstring reconstruction of the anterior cruciate ligament (ACL) using a transtibial (TT) or an anteromedial portal (AMP) approach to drill the femoral tunnel. Methods Ninety-four patients were operated on in 2005 and 2006, and 88 (93.6%) (73 men, 15 women) were evaluated subjectively and objectively, using the Lysholm and International Knee Documentation Committee (IKDC) scores, manual maximum displacement test with a KT-1000 arthrometer (MEDmetric, San Diego, CA) and the Lachman test, and rotational instability with the pivot shift test. Degenerative changes were assessed on radiographs according to the Fairbank classification. Results The median age at operation was 29 years (20 to 43 years; SD, 5.4) in the TT group 1 and 28 years (19 to 45 years; SD, 6.1) in the AMP group 2. At the last appointment, the 2 groups had similar results for the Lysholm and IKDC scores (primary outcome). Patients who underwent the AMP approach had less glide pivot shift ( P = .42) and Lachman ( P = .47) phenomena, with no statistically significant intergroup difference. Radiography showed fewer, but not significantly different, degenerative changes in the AMP ACL reconstruction group at final follow-up ( P = .47). Conclusions In our series, ACL reconstruction using a femoral tunnel drilled through an AMP provided better rotational stability and anterior translation than drilling the femoral tunnel using the TT technique. This difference likely is not relevant from a clinical and functional viewpoint. The 2 groups of patients were not significantly different in terms of development of degenerative changes seen radiographically at a minimum follow-up of 5 years. Level of Evidence Level III, retrospective comparative study.

Epigenetic control of skin differentiation genes by phytocannabinoids

Abstract: BACKGROUND AND PURPOSE Endocannabinoid signalling has been shown to have a role in the control of epidermal physiology, whereby anandamide is able to regulate the expression of skin differentiation genes through DNA methylation. Here, we investigated the possible epigenetic regulation of these genes by several phytocannabinoids, plant-derived cannabinoids that have the potential to be novel therapeutics for various human diseases.