University of Defence

About: University of Defence is a education organization based out in Brno, Czechia. It is known for research contribution in the topics: Signal & Nerve agent. The organization has 3562 authors who have published 3600 publications receiving 33696 citations. The organization is also known as: University of Defense.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Abstract: In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

The comet assay: topical issues

Abstract: The comet assay is a versatile and sensitive method for measuring single- and double-strand breaks in DNA. The mechanism of formation of comets (under neutral or alkaline conditions) is best understood by analogy with nucleoids, in which relaxation of DNA supercoiling in a structural loop of DNA by a single DNA break releases that loop to extend into a halo-or, in the case of the comet assay, to be pulled towards the anode under the electrophoretic field. A consideration of the simple physics underlying electrophoresis leads to a better understanding of the assay. The sensitivity of the assay is only fully appreciated when it is calibrated: between one hundred and several thousand breaks per cell can be determined. By including lesion-specific enzymes in the assay, its range and sensitivity are greatly increased, but it is important to bear in mind that their specificity is not absolute. Different approaches to quantitation of the comet assay are discussed. Arguments are presented against trying to apply the comet assay to the study of apoptosis. Finally, some of the advantages and disadvantages of using the comet assay on lymphocyte samples collected in human studies are rehearsed.

The selection of transport and handling resources in logistics centers using Multi-Attributive Border Approximation area Comparison (MABAC)

Abstract: Application of a new MCDA technique called MABAC.The MABAC sensitivity analysis.Five other MCDA techniques were tested under the same conditions.The MABAC showed stability and other techniques. This paper presents the application of the new DEMATEL-MABAC model in the process of making investment decisions on the acquisition of manipulative transport (Forklifts) in logistics centers. The DEMATEL method was used to obtain the weight coefficients of criteria, on the basis of which the alternatives were evaluated. The selection of criteria for evaluating Forklifts was based on an analysis of available literature. The evaluation and selection of Forklifts was carried out using a new multi-criteria method - the MABAC (Multi-Attributive Border Approximation area Comparison) method. This paper presents a practical application and a sensitivity analysis of the MABAC method. The sensitivity analysis was conducted in three stages. In the first stage, a stability analysis was carried out on the solution reached by the MABAC method, depending on changes made to the weights of the criteria. In the second and third stages, a consistency analysis of the results from the MABAC method was carried out depending on both the changes in the measurement units in which the values of individual criteria are presented and on the formulation of the criteria. The SAW, COPRAS, TOPSIS, MOORA and VIKOR methods were tested under the same conditions. Based on the results obtained, it was shown that the SAW, COPRAS, TOPSIS, MOORA and VIKOR methods do not meet one or more of the conditions set, while the MABAC method showed stability (consistency) in its solutions. Through the research presented in this paper, it is shown that the new MABAC method of multi-criteria decision-making is a useful and reliable tool for rational decision-making.