Showing papers in "Analytical Biochemistry in 2007"

TL;DR: The cell is deemed to be the most basic structural and functional unit of all living organisms and often is called a ‘‘building block of life’’ and playing a critical role in generating energy in the eukaryotic cell.

TL;DR: The preliminary study shows that the nonenzymatic sensor has synergistic electrocatalytic activity to the oxidation of glucose in alkaline media and has good reproducibility and long-term stability and is interference free.

TL;DR: It is demonstrated that sample pretreatment with urease dramatically alters the metabolome composition apart from removal of urea, which will likely lead to clinically applicable assays for earlier diagnosis of RCC, as well as other malignancies, and thereby improved patient prognosis.

TL;DR: Analysis of the four redox couples throughout Arabidopsis rosette development showed that redox states were relatively constant but that total pools of NAD, glutathione, and ascorbate were significantly modified by day length and developmental stage.

TL;DR: This study optimized a magnetic-bead-based platform amenable to high-throughput peptide capture and demonstrated that antibody capture followed by mass spectrometry can achieve ion signal enhancements on the order of 10, sufficient for quantifying biomarkers in the physiologically relevant ng/mL range.

TL;DR: Primary study indicated that Au-PtNps/CNTs had a better synergistic electrocatalytic effect on the reduction of hydrogen peroxide than did AuNPs/C NTs or PtNPs / CNTs at a low applied potential window.

TL;DR: This study represents a unique approach in that MS/MS data were used to identify and confirm the oligosaccharide structures of a protein.

TL;DR: A strategy for the development of an advanced analytical platform that allows the comprehensive analysis of microbial metabolomes and its application to the analysis of the metabolome composition of mid-logarithmic E. coli cells grown on a mineral salts medium using glucose as the carbon source is presented.

TL;DR: The use of fusion constructs of small proteins with multimeric EGFP alone is not adequate to prove nuclear import processes, because even EGFP homotetramers and homohexamers are found in the nuclei of the five analyzed mammalian cell lines.

TL;DR: Evidence is provided that it is feasible to monitor two distinct activities at 37 degrees C with these novel thermostable proteins, and model studies established that the luciferases could be detected at the attomole level and six orders of magnitude higher.

TL;DR: A unique, late-eluting "basic peak" was observed by weak cation exchange-HPLC (WCX) for a recombinant monoclonal antibody (mAb) sample, and the occurrence of C-terminal alpha-amidation in mAbs has not been reported previously.

TL;DR: Ratiometric measurement of viable and dead cells in the same sample provides an internal control that can be used to normalize data from other cell-based assays, and can be multiplexed further with other assays in a homogeneous format.

TL;DR: This method was used to quantitatively profile regional differences in nine discrete rat brain regions for AEA, 2-AG,2-LG, OEA, PEA, noladin ether, virodhamine, and arachidonyl glycine.

TL;DR: A novel and robust quenching method for microbial cell cultures based on cold glycerol-saline solution as the quench agent that prevents significant leakage of intracellular metabolites and, therefore, permits more accurate measurement of intrACEllular metabolite concentrations in microbial cells.

TL;DR: This review outlines some of the general principles that are important when developing and using fluorochrome-labeled optical contrast agents for oncology investigations in animals.

TL;DR: A method has been developed, using tandem MS and neutral loss scanning, to quantitatively analyze changes in those glyceryl ester molecular species containing identical fatty acyl groups, and a 50:1-TAG containing 18:1 was found to increase significantly over a 24-h time course after Kdo(2)-lipid A exposure.

TL;DR: A unique bimetallic, nano platinum with nano gold on nafion incorporated with functionalized multiwall carbon nanotubes (f-MWCNTs-NF-PtAu) composite film was developed by the potentiostatic method and exhibits promising efficient catalytic activity towards the oxidation of mixture of biochemical compounds.

TL;DR: Liquid chromatography-electrospray tandem mass spectrometry is suitable for rapid measurements, and the reported urinary values in children will help to develop a pediatric reference range for clinical use.

TL;DR: An on-line reversed-phase (RP) high-performance liquid chromatography/mass spectrometry (MS) method has been developed for profiling and characterizing N-glycans from recombinant immunoglobulin G antibodies, achieving good resolution of all major glycans and segregates glycan types to different regions of the chromatogram.

TL;DR: It was revealed that KS, HS, and DS in human serum and plasma were digested to each disaccharide by keratanase II, heparitinase, and chondroitinase B, respectively, and this method is sensitive and reproducible, and would be useful for clinical diagnosis.

TL;DR: Novel fluorescent substrates for the human ADAM family members ADAM17, ADAM10,ADAM8, and ADAM12 that have good specificity constants and are useful for high-throughput screening of inhibitors are described.

TL;DR: Site-directed biotinylation of antibodies at the hinge region was developed to immobilize antibodies in an oriented manner via biotin-streptavidin linkage by reacting activated biotin with F(ab')(2) fragment prepared by enzymatic cleavage.

TL;DR: Although many slide types produce useful data, glass slides coated with aldehyde silane, poly-l-lysine, or aminosilane consistently produce superior results in the sandwich ELISA microarray analyses the authors performed.

TL;DR: Mechanistic studies showed that the overall CySH oxidation is controlled by the oxidation of the CyS(-) electroactive species, and CILE manifests a good electrocatalytic activity toward oxidation of l-cysteine.

TL;DR: Using the substrate's extinction coefficients at excitation and emission wavelengths, the inner filter effect can be modeled during curve fitting for more accurate Michaelis-Menten parameters and the economies of the progress curve approach make accurate k(cat) and K(m) more accessible from fluorescence measurements.

TL;DR: A new DNA electrochemical biosensor is described for electrochemical impedance spectroscopy (EIS) detection of the sequence-specific DNA related to PAT transgene in the transgenic plants.

TL;DR: The results show that OP pesticides and nerve agents bind covalently to human albumin at Tyr411, and the presence of Tyr411 on an exposed surface of albumin suggests that an antibody response could be generated against OP-albumin adducts.

TL;DR: The prepared biosensor is economic and disposable, due to the low-cost ITO film electrode obtained from industrial mass production, but also is capable with good detection precision, acceptable accuracy, and storage stability for the fabrication in batch.

TL;DR: A quick and highly sensitive assay for protein thiol and dithiol quantification using the reducing agent sodium borohydride and the thiol reagent 4,4'-dithiodipyridine (4-DPS).