Showing papers in "Biochemical Pharmacology in 1968"

TL;DR: The hypothesis that in the enzyme prepared, the MAO is a binary system of enzymes each of which has a detectably different sensitivity to this particular inhibitor, is put forward and evidence after dialysis supports this hypothesis.

TL;DR: It is suggested that deamination, reduction, and subsequent conjugation with sulfate is the primary route of metabolism of normetanephrine in rat brain and that norepinephrine is also metabolized to this sulfate conjugate.

TL;DR: Murine leukemic cells, L5178Y, inhibited by a low level of cytosine arabino-side for 6 hr could be rescued by deoxycytidine, and the incorporation into RNA could be correlated with irreversible inhibition of cell reproduction.

TL;DR: Kinetic studies indicate that while platelets and plasma MAO are distinct from that of liver mitochondria, the platelet and liver enzymes are quite similar, particularly in the characteristics of their response to inhibitors.

TL;DR: Results of differential centrifugation of bovine pineal gland and of rat cerebellar homogenates indicated that most of the adenyl cyclase is particulate, being in the mitochondrial and microsomal fractions, whereas the majority of the phosphodiesterase is soluble.

TL;DR: The results are in accord with the view that CCl4 acts through an active metabolite, but they do not necessarily imply that the Active metabolite is formed by a cytochrome P-450 enzyme.

TL;DR: Lauric acid inhibits the binding of phenylbutazone-14C and warfarin- 14C by competing with these compounds for the same binding site on the protein.

TL;DR: The weight loss was the best index of withdrawal; it was dose-dependent and could be quantified, and the increase in brain NE after morphine is peculiar to this drug and is not a characteristic of the drug class.

TL;DR: Treatment with drugs which precipitate acute attacks in human hepatic porphyria resulted in a marked increase of the level of cytochromes without showing a decrease phase, whereas the activity of ALA synthetase remained at a level 1.5–2 times higher than normal without an increase of porphyrin.

TL;DR: Rejection episodes in human kidney transplants are associated with the formation of platelet aggregates in the kidney and can be reversed by administration of anti-inflammatory drugs such as cortisone and phenylbutazone.

TL;DR: Tetrahydrouridine markedly inhibited the deamination of ara-C at nontoxic doses in two dogs and two rhesus monkeys, consistent with a model of partial (or regulatory-type) inhibition.

TL;DR: There was no temporal correlation between the alteration of brain 5HT metabolism and the onset of an aggressive behavior and female mice and two strains of rats which do not become aggressive after prolonged isolation did not show any change inbrain 5HT turnover rate.

TL;DR: A schematic model in the biosynthesis of catecholamines is proposed, suggesting the existence of an important step between decarboxylation and β-hydroxylation, and in the course of uptake experiments dopamine was shown to be most rapidly taken up into the granules.

TL;DR: The dopamine-s-hydroxylase activity in the subcellular fractions measured with and without the addition of Cu2+ confirms previous reports that the enzyme is largely localized in the catecholamine storage vesicles and indicates that the enzymes is more easily solubilized than was previously thought.

TL;DR: Nerve-ending particles (synaptosomes) isolated from rat brain were studied as a model system for synaptic function with particular reference to the ionic conditions required for uptake of norepinephrine.

TL;DR: These experiments avoid many of the pitfalls and errors imposed by infusing exogenous labeled noradrenaline where one measures the metabolic products of circulating exogenous norad renaline rather than the natural occurring norad Renaline.

TL;DR: Observations suggest: (1) that very pronounced changes in the normal metabolism of 5-HT may be produced by treatments that modify total endogenous stores only slightly; (2) that the elevation of 4-HT by stress may be associated with an increase, not with a decrease, in the metabolism of serotonin; and (3) that an appreciable proportion of the 5-HIAA formed under normal non-stressed conditions may be derived from newly synthesized 5- HT.

TL;DR: dl - Threo -DOPS rapidly replenishes norepinephrine levels in reserpine-treated mice, but does not cause the awakening effect shown by l -DOPA under the same conditions.

TL;DR: Evidence has been presented that there are at least three forms of mitochondrial MAO in the mouse capable of deaminating kynuramine and it is suggested that some of the pharmacological effects of the MAO inhibitors may depend on specific inhibition of single forms.

TL;DR: It is suggested that protein bound glutathione may represent a reservoir of physiological radiation protector and be of significance or the radio protective action of cysteamine.

TL;DR: The rat liver lysosome seems to to be heterogeneous, pharmacologically, and the dose-response relationship for enzyme release from the heavy lysOSomes, was studied.

TL;DR: The sex difference in the metabolism of drugs by rat liver microsomes is not entirely explained by differences in the amount of enzyme, but for the N-demethylation of ethylmorphine there is also a sex differencein the apparent affinity constant as well as the maximal velocity.

TL;DR: Gas chromatographic analysis of blood and brain extracts from rats anesthetized with 1,4-butanediol revealed that BD was metabolized to γ-hydroxybutyrate (GHB), which is taken to support the view that GHB is the active metabolite involved in the production of the “sleep” induced by BD.

TL;DR: The metabolism of hexobarbital by the isolated perfused rat liver was reduced after intoxication with CCl 4 and may have been responsible, in part, for the enhanced action of the barbiturate.

TL;DR: Parallel histochemical and biochemical microassay procedures have been used to confirm that a large increase in endometrial monoamine oxidase occurs in the late secretory phase of the human menstrual cycle and no such variation was observed during the rat oestrus cycle.

TL;DR: It is indicated that in intact postganglionic sympathetic nerves, reserpine inhibits the synthesis of noradrenaline by inhibiting the uptake of the precursor dopamine into the granular vesicles which contain the enzyme necessary for its ultimate conversion to norad Renaline.