Showing papers in "Clinica Chimica Acta in 2000"

TL;DR: The improved procedures for the assay of different magnesium states are useful in understanding the role of magnesium in health and disease.

TL;DR: The effect of interactions among tumor cells, stromal cells, and the extracellular matrix on the regulation of protease expression is discussed and the role of inhibitors and novel protease-based drugs for clinical use are discussed.

TL;DR: A simple and reproducible microtiter plate assay for measuring superoxide dismutase (SOD) activity is described, applicable for chloroform-ethanol red cell extracts as well as tissue homogenates without high-speed centrifugation.

TL;DR: Increased free radical generation, especially superoxide anion in leukemia patients and increased antioxidant defence enzymes, which is an adaptive protective response, are indicative of mild oxidative stress.

TL;DR: This review shows that the need of proteinases for bone resorption is critical not only because they are required for the solubilization of bone matrix, but alsoBecause they are key components of the mechanism that determines where and when bone Resorption will be initiated.

TL;DR: Measurements of calprotectin in fecal samples were accurate and reproducible, no interference with foods or relevant oral pharmaceuticals or nutraceuticals was found, and no significant difference between spot measurements was found when five samples from each of 47 stools were measured.

TL;DR: Antioxidant activity was measured by oxygen-radical absorbing capacity (ORAC) using 2, 2'-azobis(2-amidinopropane) dihydrochloride (AAPH) as a peroxyl radical generator to assess the antioxidant activity of caffeine and its metabolites at physiological concentrations.

TL;DR: Significant higher cystatin C concentrations were found in asthmatic patients compared to controls which suggests its role in the pathogenesis of asthma, and supports the need for the evaluation of cyStatin C as a marker of glomerular filtration rate determination in asthma.

TL;DR: In vitro studies revealed that elevated glucose concentrations increase collagen synthesis similar to the in vivo situation and showed that hyperglycemia may be toxic either by non-enzymatic reaction of glucose with proteins and subsequent formation of advanced glucosylation end products or by increased metabolism leading to increased oxidative stress and activation of protein kinase C resulting in increased production of cytokines.

TL;DR: Results of studies, mostly performed in the laboratory, aimed at understanding the effect of cathepsin D overexpression on metastasis and the molecular mechanisms involved are summarized to guide in the development of new therapies aimed at inhibiting the deleterious effect of overexpressed cathePSin D.

TL;DR: A dipstick test plus an optical strip reader that can measure urine protein, albumin, and creatinine and calculate the appropriate ratios provides a better screening test for albuminuria or proteinuria than one measuring only albumin or protein.

TL;DR: It is shown that that three compounds; pioglitazone, metformin and pentoxifylline are also inhibitors of glycation, showing that there are no currently available medications known to block AGE formation.

TL;DR: The compartmentalization and the elimination rate of PEth in human whole blood was determined by an improved HPLC technique, with evaporative light-scattering detection and a weak significance was observed in the correlation ofPEth at day 1 and half-life values of the same subjects.

TL;DR: The obtained results indicate an enhanced generation of reactive oxygen species and impairment of cellular and extracellular antioxidant systems potential in patients with Graves' disease and the attainment of euthyroid state led to an improvement in oxidative stress indices and antioxidant potential parameters.

TL;DR: Cystatin C, a low molecular weight protein which can be measured by light scattering immunoassay, possesses many of the attributes required of the ideal GFR marker and demonstrates a better correlation with the reference procedure than serum creatinine.

TL;DR: Urine particle flow cytometers have improved count precision and accuracy compared to visual microscopy and offer significant labor saving and the absence of an internationally recognized reference measurement procedure is a serious drawback to their validation.

TL;DR: The limit of detection of the method was determined as 2.5 micromol/l, which enabled sulphide levels in gut contents and whole blood samples obtained from humans to be accurately determined.

TL;DR: CTnI and cTnT were found to be early markers for diagnosing iso-induced myocardial damage in comparison with CK and LD and can be used as easily measurable target parameters for detection of cardiotoxic and/or cardiodegenerative effects in rats.

TL;DR: The results suggest that the simultaneous increase of the number of ejaculated spermatozoa and the mitochondrial enrichment of citrate synthase and complex II are both parallel responses to the same regulatory events.

TL;DR: It is suggested that the plasma BNP concentration may be an inexpensive, simple and useful marker of RV overload and end-stage CRD death in CRD patients, and this preliminary results need to be confirmed in a large series of CRD Patients.

TL;DR: Although impaired antioxidant defence may be responsible for recurrent abortions, the recurrent abortions may also result in oxidative stress and depletion and weakness of antioxidant defence.

TL;DR: Analysis of the molecular mechanisms underlying its marked overexpression in malignant tumors has allowed to identify different cytokines, growth factors and tumor promoters with ability to up-regulate collagenase-3 expression in tumors, or in stromal fibroblasts surrounding epithelial tumor cells.

TL;DR: Urine specimens submitted for trace metal analysis from patients residing in the United States are used to calculate representative ranges for 25 urinary trace elements, and to compare them to reference values taken from the literature.

TL;DR: Hematological parameters and blood markers that indicate oxidative stress, such as lipid peroxides (LPO), reduced and oxidized glutathione (GSH, GSSG), superoxide dismutase (SOD) and glutATHione peroxidase (GSF), were measured in 18 marathon runners before, immediately after the race, and after 8 days of rest to points to PMN as an important oxygen radical source established in the runners' blood.

TL;DR: A spectrophotometric assay for serum platelet-activating factor acetylhydrolase (PAF-AH, EC 3.1.47.), which does not require radioisotopes and is applicable to an automatic analyzer, should be particularly useful for clinical diagnostics.

TL;DR: The role of the primary antioxidant defence system, constituted by the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase, protecting cells from toxic oxygen is studied, and how the imbalance of reactive oxygen species is related to inflammation in allergic diseases by affecting immune cells is analyzed.

TL;DR: The method of zero-crossing-point first-derivative spectrophotometry was applied to calcium oxalate species quantitation and revealed to be easy, accurate, precise and very well adapted to routine laboratories.

TL;DR: Two commercial TRAP assays were evaluated, an electrophoretic and an ELISA assay formats, using cultured cells and human tumor samples, and found that both had a limited linearity from 250 to 5000 tumor cells, with a similar intra-assay variation.

TL;DR: Markers of inflammation appear to offer risk prediction information that is independent of, and possibly complementary to, traditional risk factors for the development of cardiovascular disease.

TL;DR: Either plasma or whole blood samples can be accepted for PT testing up to 24 h and for aPTT testing up for 12 h only, when transported either at RT or at 4 degrees C.