Showing papers in "Journal of Chromatography B: Biomedical Sciences and Applications in 1982"

TL;DR: Both ion-pair reversed-phase and ion-exchange liquid chromatography have been used for the catecholamines and the method has been validated against radioenzymatic assay and the choice of method is discussed.

TL;DR: A review of the current analytical methodology and recent applications of HPLC in the field of catecholamine metabolism can be found in this paper, where the authors discuss the impact of this technique on biochemical investigations and its future potential in clinical laboratories.

TL;DR: This enzymatic hydrolysis--HPLC method is the best described to date for quantitative determination of the nucleoside composition of tRNAs and has already provided important information for investigation of the role of modification in the function of RNAs.

TL;DR: A rapid and efficient high-performance liquid chromatography procedure for the separation of phospholipids using isocratic elution and UV detection at 203 nm, which is suitable for the analysis of phosphotidylcholine in tissue extracts.

TL;DR: The precision of the method has been investigated by determining the reproducibility at different levels of nicotine and cotinine within the working ranges, for both 1-ml and 0.1-ml samples of plasma.

TL;DR: Using this HPLC method, urinary neopterine related to creatinine was determined for 148 healthy male adults, 146 healthy female adults, and 60 healthy children, with the aim of reducing variations due to fluctuating urinary concentrations.

TL;DR: A comparative study of angiotensin-converting enzyme activity in plasma, kidney and lung of five experimental animals showed a high degree of variation from species to species.

TL;DR: The method is applicable to both neurochemical research and routine clinical analysis and the limit of quantitative detection of the method was found to be approximately 30 pg per injection for individual catecholamines.

TL;DR: A method using reversed-phase ion-pair liquid chromatography with dual detection was developed for the simultaneous determination of the S-adenosylmethionine (SAM) analogues and the natural polyamines in one single run by direct injection of tissue extracts.

TL;DR: A simple and rapid method for separation of six major phospholipids and four major neutral lipids from cell extracts by one-dimensional preadsorbent thin-layer chromatography was developed and is suitable for the separation of relatively large amounts of radiolabeled and non-radiolabeling lipids and free fatty acids from extracts of biological fluids, tissues, or cells maintained in monolayer culture.

TL;DR: This work has designed a method to extract leukotrienes from biologic fluids using commercially available silica mini-columns requiring minimal preparation and using a sensitive and reproducible HPLC technique.

TL;DR: The stability of the cephalosporins in plasma, urine and water and the reproducibility and accuracy of the methods are reported.

TL;DR: Proteolipids from adult rat brain subcellular fractions were purified by a one-step procedure involving chromatography through Sephadex LH-60 eluted with an acidified chloroform-methanol mixture, indicating that the proteolipid apoproteins purified by this method do not adsorb hydrophobic low-molecular-weight compounds.

TL;DR: The sensitivity limits of detection are improved significantly for acetaminophen and all of the metabolites except the sulfate and glucuronide, by amperometric detection (electrochemical) of the same sample as it elutes from the UV detector.

TL;DR: Two methods for the extraction of morphine from cerebrospinal fluid or plasma with quantitation by high-performance liquid chromatography with electrochemical detection were compared for accuracy, precision and ease of preparation.

TL;DR: A method for the identification and quantitation of nucleotide pools in lymphocytes and leukemic blasts using anion-exchange high-performance liquid chromatography using a pH and concentration gradient consisting of several linear steps is described.

TL;DR: Tryptophan, serotonin, N-acetylserotonin, melatonin, 5-hydroxyindoleacetic acid and 5-Hydroxytryptophol have been determined in rat and human pineal glands.

TL;DR: This review primarily deals with methods for separations of hemoglobins and considers electrophoretic methods as well as those involving isoelectric focusing and chromatography.

TL;DR: The method has been successfully applied to the analyses of over 1200 blood samples from four 13-cis-RA clinical studies and the mean intra- and inter-assay variabilities for all four compounds were 6% or less.

TL;DR: A sensitive and selective high-performance liquid-chromatographic assay for ibuprofen and its major metabolites in biological fluids is described.

TL;DR: A rapid, simple and sensitive sample cleanup procedure and high-performance liquid chromatographic (HPLC) assay for both bisantrene and mitoxantrone, allowing us to document a prolonged terminal phase plasma half-life for both drugs.