Showing papers in "Journal of Immunological Methods in 1974"

TL;DR: Centrifugation of heparinized human blood on discontinuous gradients of Ficoll-Hypaque resulted in the simultaneous separation of mononuclear leukocytes, granulocytes, and erythrocytes with high recovery of each cell type.

TL;DR: An improved procedure for detection of human T lymphocytes using sulfhydryl-treated sheep erythrocytes is described, and the data reported confirm the presence of ‘double marker’ lymphocytes in all normals and chronic lymphocytic patients studied.

TL;DR: Restoration experiments with peritoneal cells and 2-mercaptoethanol show that normal and antigen-primed T lymphocytes with helper function and B lymphocytes which are precursors of antibody-forming cells are present in substantial quantity in the effluent population.

TL;DR: The buffering with piperazine of the chromic chloride-antigen reaction mixture used for coating red cells with antigen results in a useful improvement in the technique.

TL;DR: Development of a simplified and versatile microculture harvesting device which can process cell precipitates as well as cell supernatants, can be made by any machine shop, and has proven extremely useful in a laboratory of immunology.

TL;DR: The use of FUdR-augmented 125 IUdR uptake represents a rapid and reliable method in the quantitation of mitogenic responses in human and chicken lymphocyte responses to phytohemagglutinin.

TL;DR: It is demonstrated that fixation of tissues and cells with 1% paraformaldehyde does not seriously affect tissue and membrane-bound antigens and addition of glutaraldehyde in very low concentrations improved the morphological conditions required for immunoelectron microscopical studies.

TL;DR: A sensitive, quantitative immunofluorescence method has been developed, using proteins covalently coupled to Sepharose beads, which was tested in the quantitative measurement of antibodies and antigens.

TL;DR: A simplified chronium-51 release assay for lymphoid cell-associated cytotoxic (killer) activity is described that uses a microtest plate for incubation instead of tubes and two modifications are described that hasten cell-to-cell contact and greatly reduce the number of target cells required.

TL;DR: Conjugates with molar ratios of antibody or Fab fragment to peroxidase being one, seem more effective, although no significant differences were noted between per oxidase conjugates of whole antibody molecules and Fab fragments.

TL;DR: The DASS-system is a promising quantitative method for the identification of a variety of antigens using the indirect immunofluorescence method and is at least as sensitive as the indirect fluorescent antibody reaction with frozen sections of the adult parasite.

TL;DR: Optimal 3 H-Tdr labelling conditions were achieved with saturation concentrations of exogenous Tdr of at least 3.0 μg/ml during culture in the most highly proliferating cultures, and it was calculated that at saturation concentrations the maximal SA should be used to provide the highest uptake.

TL;DR: Fixation with PFA under specified conditions did not diminish the fluorescence intensity of cells stained by direct or indirect immunofluorescence and decreased only slightly the reaction of anti-immunoglobulin with cell-bound fixed antibody.

TL;DR: An in vitro microassat for lymphotoxin (LT) using mouse L-929 fibroblasts as target cells grown in the wells of microculture plates is described and the advantages and limitations of the microassay are shown using LT-containing supernatant fluids from human lymphoblast cell lines and PHA-P- and Pha-M-stimulated human lymphocytes.

TL;DR: A method of blocking the non-specific fluorescent staining of granulocytes, particularly eosinophils, using prior treatment with Lendrum's phenol chromotrope 2-R stain, which has been successful with anti-immunoglobulin antisera in rabbit, chicken, and human tissues.

TL;DR: By reacting EA with serum in the presence of TTHA, active and stable cellular intermediate of immune hemolysis, EAC14, was successfully prepared and this method does not require isolated components of complement and is applicable to prepare EAC 14hu.

TL;DR: A modified assay is described for spontaneous sheep red cell-rosette formation by human T lymphocytes, finding five minutes incubation at 37°C followed by incubation of the lymphocyte-SRBC pellet at 4°C for 2 hr provides optimal conditions for high rosette numbers.

TL;DR: A motor-driven cell-rupturing pump appears to be at least as effective as any other available device for cell rupturing, and is small, versatile, and simple to use.

TL;DR: An improved 51Cr release assay for cytotoxic lymphocytes has been developed that combines the technical advantages of a titration tray method with a modification which enables a more quantitative measure of the cytotoxicity of sensitized cell preparations in a shorter time.

TL;DR: Snake venom components were measured by solid-phase radioimmunoassay and Circulating venom was detected in cases of human and experimental envenomation and for indirectly assaying antiveness.

TL;DR: Factors that affect the degree of stimulation in allogeneic cultures and those that contribute to the variability of the assay are defined.

TL;DR: Where cell destruction by cytolytic viruses might prevent in situ fixation of cell monolayers, a rapid and simple RIA procedure is described which utilizes imitation pearls as antigen adsorbents.

TL;DR: Dimethylsulfoxide (DMSO) dissolves tetramethylrhodamine isothiocyanate (TRITC) as well as fluorescein isotho-FITC rapidly and completely, which facilitates the labelling of antibodies with fluorochromes with special reference to TRITC, which cannot be completely dissolved in other non-denaturing agents.

TL;DR: It was demonstrated that the ratio fluorescence/number of bound fluorochrome molecules obtained for more heavily stained beads may be extrapolated to lightly stained beads, and by comparing beads and plasma cell fluorescence, the latter may be expressed in number of fluorochrom molecules bound to aminoethyl-Sephadex.

TL;DR: Aleutian disease virus (ADV) antigen was prepared by fluorocarbon extraction from ADV-infected mink tissue and it is suggested ADV is a member of the parvovirus group.

TL;DR: Marked localization occurred consistently in the immune animals 24 and 48 hr after the challenge; this was shown to be a more sensitive index of the cellular manifestation of the immune reaction than the increase in the thickness of the ear.

TL;DR: It was found that for lymphocytes stored at −180°C demonstration of the full number of immunoglobulin-bearing lymphocytes in chronic lymphatic leukaemia was critically dependent on the concentration of the indicator suspension of sensitized red cells.

TL;DR: The introduction of a direct enumeration of the cells in the lower compartment of a chamber specially convenient for this purpose improved the reproducibility and normal values of reference for clinical interpretation were established, as well as the kinetics of migration as a function of time.

TL;DR: The principle of a novel method for the immunological assay of haptens, based on nephelometry, has been tested, using ϵ-dinitrophenyl-lysine and progesterone as model substances, and is proposed to call this method the nephelometric inhibition immunoassay (NINIA).

TL;DR: It was found that those antibodies that required the highest concentrations of thiocyanate to dissociate them from their antigen, were also those that formed aggregates with the most elevated nephelometric effect.