Showing papers in "Journal of Lipid Research in 1973"

TL;DR: The o-phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation and is three times more sensitive than the FeCl(3) determination.

TL;DR: A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described and it followed Beer's law in the range of 1-10 micro g ofospholipid phosphorus.

TL;DR: In the intact rat, liver appeared to be the major source of heparin-released TGLA measured at pH 8.6 with triolein substrate, and it was further shown that both hepatic and extrahepatic lipases catalyzed hydrolysis of triglyceride-rich lipoproteins.

TL;DR: A new assay for 3-hydroxy-3-methylglutaryl CoA reductase (mevalonate:NADP oxidoreductase [acylating CoA], EC 1.1.34) is based upon the measurement of released coenzyme A (SH) during the reduction of 3-Hydroxy- 3- methylglut Daryl CoA to mevalonates.

TL;DR: A kinetic analysis is presented for the dependence of one form of phospholipase A(2) from cobra (Naja naja) venom on the presence of the nonionic detergent Triton X-100 for its activity towards egg phosphatidylcholine and synthetic dipalmitoyl glycerophosphorylcholine as substrates.

TL;DR: It is concluded that the composition of the bile acids pool influences the hepatic concentrations of the rate-controlling enzymes of bile acid synthesis.

TL;DR: The results suggest that nascent VLDL of gut origin acquires group III peptides from other lipoproteins that penetrate lymph from plasma.

TL;DR: Feeding a diet high in cholesterol resulted in a marked increase in the concentration of lipoproteins of density less than 1.006, and a new lipoprotein fraction appeared between d 1.063-1.21, which contained immunologically demonstrable low density and high density lipop Protein.

TL;DR: The results of this study suggest that interfascicular adipose tissue is a late developing depot and thathyperplasia is still an active process in this depot at 14 months of life, whereas hyperplasia appears to be nearly complete in the subcutaneous and perirenal depots of bovine animals by about 8 months oflife or shortly thereafter.

TL;DR: It was found that these levels had a wide range in normal human subjects but that the ratio of RBC to plasma tocopherol was relatively constant and equal to 0.18, uncorrected, and when both RBC and plasma values were corrected to 100% recovery.

TL;DR: The activities of the selected lipogenic enzymes (expressed on an adipose cell basis) increased markedly at weaning and again during the rapid increase in percentage of body fat between 3.5 and 5 months.

TL;DR: Results suggest that ethanol inhibited alpha-ketoglutarate dehydrogenase in the mitochondria of hepatocytes by elevating the mitochondrial NADH:NAD ratio, resulting in an increased availability of long-chain free fatty acids and enhances esterification, leading to accumulation of liver triglyceride.

TL;DR: The fate of (125)I-labeled apolipoproteins was studied in vivo in rats that had received intravenous injections of ( 125)I -labeled human HDL, LDL, and VLDL and the half-lives of the different HDL apoproteins were not identical.

TL;DR: Results demonstrate that uptake of short-chain fatty acid monomers is rate limited by the lipid cell membrane but diffusion through the unstirred water layer becomes increasingly rate limiting as the chain length increases.

TL;DR: The long-term turnover of plasma cholesterol was examined in six men injected intravenously with [4-(14)C]cholesterol and the results indicate that the turnover curves of all six subjects conformed to, and could be satisfactorily described by, a three-pool model.

TL;DR: It was demonstrated that the formation of PGE(2) from arachidonic acid by the microsomal fraction from human skin could be inhibited by polyunsaturated fatty acids, suggesting a possible regulatory role of fatty acids released from endogenous phospholipids on prostaglandin synthesis in this tissue.

TL;DR: Results confirm previous observations indicating that the sympathetic nervous system and adrenalin release from the adrenal medulla participate in the production of hyperglycemia by 2-deoxyglucose and provide evidence for the existence of centers that can specifically influence mobilization of free fatty acids through a direct activation of the sympathetic fibers of adipose tissue without intervening in glucose homeostasis.

TL;DR: A new procedure for the preparation of methyl esters from free fatty acids under mild conditions was investigated, and neither artifact formation nor loss of unsaturated acids was observed.

TL;DR: The results suggest that cholesterol feeding leads to an increase in biosynthesis of lipoproteins with d < 1.063 g/ml, and from the apoprotein composition, it is concluded that the very low density fraction present in cholesterol-fed animals is more structurally related to low density lipiproteins than to the veryLow density lipoprotein isolated from control animals.

TL;DR: The data suggest that an important action of this hormone is to decrease the synthesis of the protein moiety of the VLDL; this may contribute to the hypolipemic action of glucagon by introducing a limitation in hepatic lipoprotein production.

TL;DR: A mixture of isomers of methylhexadecanoic acid was isolated from glycerolipids of brain, spinal cord, and sciatic nerve of a patient who died from methylmalonic aciduria, a disease in which vitamin B(12) is not converted to deoxyadenosyl B( 12), and data indicated that the points of methyl branching are located predominantly on the even-numbered carbon atoms.

TL;DR: The mitochondrial lipids from avocado fruit, cauliflower buds, and potato tubers, and the lipids of chloroplasts isolated from avocado Fruit and from cauliflower leaves were identified and the concentrations were determined and the lipid composition was compared with that of beef heart mitochondria.

TL;DR: The conditions for the preparation of conjugated bile acids by means of N-ethoxycarbonyl-1,2-dihydroquinoline are described and conjugation is obtained via the intermediary formation of mixed carbonic-carboxylic acid anhydrides.

TL;DR: A survey of differences in composition and metabolism of myelin from five areas of the central nervous system was made in brain and spinal cord slices of the rat from 20 days to 20 months postnatal age, showing a trend of chemical maturity in agreement with the anatomical observations that myelination begins in the hindbrain and proceeds rostrally.

TL;DR: The results indicate that dietary sterols and bile acids both play a role in the regulation of intestinal HMG CoA reductase, and are associated with an increase in sterol concentration within the intestinal crypt cells.

TL;DR: It would appear that levels of pancreatic lipase are increased when the fat content of the diet is raised from about 5% to 15-22%, but that little or no additional increase in lipase levels can be attained by any further increase in the amount of dietary fat.

TL;DR: Feeding a high fat diet for 24 days increased GPAT activity per milligram of protein to an extent similar to that observed after chronic ethanol administration, however, the increase was much greater after ethanol.

TL;DR: Fat tissue is a major cholesterol storage organ and may well account for the marked expansion of the slowly exchangeable cholesterol pool (pool B) observed in obesity.

TL;DR: The reported control mechanism for triglyceride biosynthesis is discussed in relation to the regulation of fatty acid uptake and release in adipose tissue and the absorption and metabolism of triglycerides by the intestinal mucosa.

TL;DR: Phospholipase was isolated (in two fractions) from Vipera palestinae venom and it was shown to possess phospholip enzyme A activity (hydrolyzing diacyl-sn-glycerophosphorylcholines, e.g., lecithin, in the 2-position).