Showing papers in "Journal of Pharmaceutical and Biomedical Analysis in 2009"

TL;DR: Steady-state and time-resolved emission spectra performed on HSA in the presence of calcofluor do not show any modification in the emission peak (330 nm) indicating the absence of any conformational change and confirming the fact that the shift observed for tryptophan residues emission in BSA is the result of fluorescence quenching of Trp-134 residue.

TL;DR: Structural analysis showed that curcumin and genistein bind HSA via polypeptide polar groups with overall binding constants of K(curcumin) and K(genistein) and the HSA conformation was altered by pigment complexation with reduction of alpha-helix and increase of random coil and turn structures suggesting a partial protein unfolding.

TL;DR: In situ derivatization-headspace GC-MS methodology has been developed and successfully applied to the determination of hydrazine in support of API batch releases and process chemistry at GlaxoSmithKline.

TL;DR: A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD) has been developed and applied to analyze the composition of ginsenosides in Korean white, red, and black ginsengs, and is expected to provide the basis for the quality assessment ofginseng products.

TL;DR: It could be concluded that UPLC-PDA-QTOFMS based chemical profiling is a powerful approach for the global quality evaluation of Radix Paeoniae as well as other herbal medicines.

TL;DR: The HybridSPE dramatically reduced the levels of residual phospholipids in biological samples, leading to significant reduction in matrix effects, and this new procedure which combines the simplicity of precipitation with the selectivity of SPE allows to obtain much cleaner extracts than with conventional procedures.

TL;DR: The proposed method was successfully applied to the real urine samples from two female patients under amitryptiline and clomipramine treatment, respectively and good linear behaviors over the investigated concentration ranges were obtained with the values of R(2)>0.998 for the target drugs.

TL;DR: A simple, rapid and sensitive method for the determination of nicotine, cotinine, nornicotine, anabasine, and anatabine in human urine and saliva was developed and was applied successfully to analysis of urinary and saliva samples without interference peaks.

TL;DR: An HPLC/DAD/ESI-MS/MS method was developed for the qualitative and quantitative analysis of the major constituents in DSS and proved sensitive, accurate and reproducible.

TL;DR: Results indicate that this new method, namely the impregnation of sampling paper with a solution of plasma-protein, formic acid and ammonium acetate, in combination with the extraction of the blood spot, is promising for therapeutic drug monitoring in stable renal transplant patients.

TL;DR: Large interindividual variability and higher exposure in patients experiencing severe toxicity support the need for therapeutic drug monitoring to ensure an optimal exposure to sorafenib.

TL;DR: Good performance in terms of recoveries, selectivity, stability, repeatability, intermediate precision, trueness and accuracy, was obtained by the developed and validated LC-MS method for simultaneous quantification of aripiprazole (ARI), atomoxetine (ATO) and their active metabolites in human plasma.

TL;DR: Gas chromatography/mass spectrometry is the preferred technique for the detection of urinary steroid androgens for drug testing in athletics and the deconjugation and the derivatisation preparative options are discussed.

TL;DR: This study ended up with a three-dimensional DOSY-COSY (1)H NMR experiment on a herbal formulation which provided both virtual separation and structural information.

TL;DR: The quick sample work-up method allows for determination of colistin A and B in clinical samples without causing hydrolysis of the prodrug Colistin methanesulfonate (CMS).

TL;DR: Both analytical techniques used in this study were able to detect biochemical changes in the serum of type 2 diabetes mellitus patients after the treatment of meetformin hydrochloride, which may be helpful to the understanding of action mechanism of metformin Hydrochloride.

TL;DR: SC CO(2)-inclusion method proved to be an effective technique for preparing solid-state inclusion complexes between beta-cyclodextrin and antifungal drugs, avoiding the use of organic solvents.

TL;DR: On-line DBS showed good repeatability with relative standard deviation (RSD) lower than 15% based on two levels of concentration (low and high) and function responses were found to be linear over the therapeutic concentration for each compound.

TL;DR: This assay was applied to therapeutic drug monitoring on patients hospitalized in intensive care and onco-hematologic units and satisfied FDA's guidance for accuracy and precision.

TL;DR: The PLE method was successfully applied to the analysis of a commercial medicated swine feedingstuff, and the results were in good agreement with those obtained using mechanical shaking or ultrasonic extraction combined with solid phase extraction (UE-SPE), previously applied in the literature for feed analysis.

TL;DR: A chromatographic method was developed for the detection and quantification of the contaminant oversulfated chondroitin sulfate (OSCS) and the impurity dermatan sulfate in heparin active pharmaceutical ingredient (API).

TL;DR: The presented HPLC-DAD method conjugated with chemometrics approach was demonstrated to be very helpful in using Dazao resources, and was possibly useful in chemotaxonomic characterization.

TL;DR: In this article, the authors proposed a quantitative isotopic 13 C NMR spectrometry at natural abundance, which enables the measurement of the 13 C content of each carbon within a molecule, thus giving simultaneous access to a number of isotopic parameters.

TL;DR: The developed column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of losartan, telmisarta, and valsartan in human urine samples of patients submitted at ARA-IIs therapy and was successfully applied.

TL;DR: A Near-Infrared analytical procedure used for screening drugs on authenticity to a Failure Mode and Effects Analysis (FMEA), including technical risks as well as risks related to human failure, enabled us to detect previously unidentified risks.

TL;DR: Comprehensive LC-MS/MS investigation on the polyphenol composition of the most common Epilobium species is reported, emphasizing the pharmaceutical importance of a uniform standardization protocol in case of their products.

TL;DR: A robust, noninvasive, real-time, on-line near-infrared (NIR) quantitative method is described for blend uniformity monitoring of a pharmaceutical solid dosage form containing 29.4% (w/w) drug load with three major excipients.

TL;DR: A reversed-phase high-performance liquid chromatography method with fluorescence detection for simultaneous determination of five anthraquinones in Rhubarb collected from nine different locations in China,Polygonum cuspidatum, Polygoni multiflori and three pharmaceutical preparations is proposed and validated.

TL;DR: Computer mapping of the possible binding sites of mangiferin revealed the molecule to be bound in the large hydrophobic cavity of subdomain IIA, which led to changes in the conformation of HSA according to synchronous fluorescence spectra, FT-IR, UV-vis and CD data.

TL;DR: HILIC/CAD technique is a useful alternative methodology, particularly for determination of counterions in low-solubility drugs, and requiring minimal training of the operating analysts.