Showing papers in "Structure in 2023"

TL;DR: Cryo-electron tomography is employed to reveal how the GV shell is formed by a helical filament of highly conserved GvpA subunits, which changes polarity at the center of the GVs cylinder—a site that may act as an elongation center.

TL;DR: In this article , an integrative multi-scale computational approach was applied to model the envelope structure of SARS-CoV-2 with near atomistic detail, focusing on studying the dynamic nature and molecular interactions of its most abundant, but largely understudied, M protein.

TL;DR: In this article , the photochemical reaction center (RC) and light-harvesting complex 1 (LH1) assemble to form monomeric or dimeric RC-LH 1 membrane complexes, essential for bacterial photosynthesis.

TL;DR: In this article , the solution-state structure of full-length Nsp1 was reported, which features an α/β fold formed by a six-stranded, capped β-barrel-like globular domain (NTD), flanked by short N-terminal and long C-terminally flexible tails.

TL;DR: In this paper , the authors reported the cryo-electron microscopic structures of the T-pilus at 3.2-Å resolution and of the plasmid pKM101-determined N-pili at 3Åresolution, and showed that different phospholipids can be incorporated into type IV secretion pili and the charge of the lumen may be of functional importance.

TL;DR: In this paper , the dual-swinging-domains model was proposed for 3-methyl-crotonyl-CoA carboxylase (MCC) catalysis and the structure of filamentous LtMCC was resolved up to 3.4, 3.9, and 7.3

TL;DR: In this article , the authors used computational protein design, yeast surface display screening, and structure-based energy scoring to identify 10 diverse new binders of BAK, including peptides from the human proteins BNIP5 and PXT1 and three non-native peptides that activate BAK in liposome assays and induce cytochrome c release from mitochondria.

TL;DR: Zhou et al. as discussed by the authors proposed a method using AlphaFold2 followed by RosettaRelax performs the best overall, and a metric containing pLDDT values and Rosetta ΔΔG is most reliable in classifying tolerated deletion mutations.

TL;DR: In this article , a 2,5-pyridinedinedicarboxylic acid (a.k.a. 1) was identified that effectively blocks the D-DT-induced activation of CD74.

TL;DR: In this paper , a method that utilizes synthetic antigen binding fragments (sABs) to isolate TOC directly from wild-type plant biomass including A. thaliana and P. sativum was introduced, which was characterized by size-exclusion chromatography coupled with small-angle X-ray scattering and isothermal titration calorimetry.

TL;DR: In this paper , the authors demonstrate that α-synuclein in vitro exhibits primarily a monomer-dimer equilibrium in nanomolar concentrations and up to a few micromolars and use spatial information from hetero-isotopic cross-linking mass spectrometry experiments as restrains in discrete molecular dynamics simulations to obtain the ensemble structure of dimeric species.

TL;DR: In this article , the T-pilus structure of Agrobacterium tumefaciens was solved by helical reconstruction, showing that it is a stoichiometric assembly of the VirB2 major pilin and phosphatidylglycerol (PG) with 5-start helical symmetry.

TL;DR: In this article , the structure of the mouse AhR PAS-B domain is resolved and a highly conserved bridge motif of AhR is responsible for the interaction with the Hsp90 dimeric lumen.

TL;DR: In this article , the E3 ligase E6AP/UBE3A has a dedicated binding site in the 26S proteasome provided by the RAZUL domain of substrate receptor hRpn10/S5a/PSMD4.

TL;DR: In this paper , the human UBR5 tetramer was shown to assemble head-to-tail to form a tetramer with all four catalytic HECT domains facing the central cavity and the N-terminal region of one subunit and the HECT of the other formed an intermolecular jaw in the dimer.

TL;DR: In this article , a new RIC-8B-deletion cell line with Gαolf and Gαs was created, and the structure of Ric-8b was determined in a cryoelectron microscopy (cryo-EM) setup.

TL;DR: In this paper , the authors combined NMR and X-ray crystallography to study the Klebsiella AP components PulL and PulM and determined the structure and associations of their periplasmic domains and described the structure of the heterodimer formed by their ferredoxin-like domains.

TL;DR: In this paper , a detailed structural dendrogram describing the evolution of capsid structural stability within the actinobacteriophages was constructed, which can be classified into 15 groups based upon their HK97-fold.

TL;DR: In this paper , the authors report the cryo-EM structures of human N-terminal acetyltransferase (hNATC) complexes with and without NAA38, together with biochemical studies.

TL;DR: A review of the current state of the art in Solid State NMR (SSNMR) is given in this article , where some interesting research studies and important technical developments are highlighted.

TL;DR: This paper proposed a robust statistical model that identifies local backbone errors in protein structures built into cryo-EM maps by combining local fit-to-density with deep-learning-derived structural information.

TL;DR: In this article , the conformational changes upon binding Akt ATP-competitive and allosteric inhibitors were analyzed using hydrogen deuterium exchange mass spectrometry (HDX-MS).

TL;DR: In this article , the authors determined several cryo-EM structures of RyR1 bound individually to ATP-γ-S, ADP, AMP, adenosine, adenine, and cAMP, but AMP is the smallest ATP derivative capable of inducing long-range structural rearrangements associated with channel activation.

TL;DR: In this article , the authors solved the crystal structure of the CAPS-1 C2PH module and showed that the C2 and PH tandem packs against one another mainly via hydrophobic residues.

TL;DR: Comparison of immunogenetic makeup, structure, and function of these three mAbs with the recently reported class-3 RBD binding mAb that potently neutralized all SARS-CoV-2 variants revealed precise antibody footprint, specific molecular interactions associated with the most potent multi-variant binding / neutralization efficacy.

TL;DR: In this paper , the PTH1R extracellular domain is shown to be highly dynamic when bound to a truncated agonist, and the structural water networks stabilize the binding of PTH.

TL;DR: In this paper , the octahedral and octameric interfaces of β-PFTs were observed to have extra densities at the octanodes and octameters, and the N-terminal region and partial β-barrel were resolved to 3.5 Å.

TL;DR: Guzior et al. as mentioned in this paper used a multidisciplinary workflow including mass spectrometry, mutagenesis, molecular dynamic simulations, machine learning, and crystallography to demonstrate substrate specificity in Lactobacillus salivarius BSH, the most abundant enzyme in human and farm animal intestines.

TL;DR: Kunci as mentioned in this paper dilakukan pada Jalan Raya Legok dengan menggunakan metode PKJI (Pedoman Kapasitas Jalan Indonesia) 2014.

TL;DR: In this article , the structure, conformation, and membrane-binding properties of Caenorhabditis elegans MRCK1 have been presented, showing that the length of the coiled-coil domain of DMPK kinases is remarkably conserved over millions of years of evolution.