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Showing papers in "Theriogenology in 1988"



Journal ArticleDOI
TL;DR: These experiments indicate that the repeated aspiration of bovine oocytes during transvaginal ultrasound scanning is possible, but more research is needed to establish optimal methods for improving the recovery rate of oocytes before this method can be used as an alternative route for the supply of oocyte for in vitro maturation and in vitro fertilization.

297 citations



Journal ArticleDOI
TL;DR: Responses indicated that thermal stress from 30 h after the onset of estrus to Day 7 post estrus increases the incidence of abnormal and retarded embryos in superovulated heifers.

223 citations



Journal ArticleDOI
Koji Niwa1, O. Ohgoda1
TL;DR: Great variation in penetration rates was observed among the different bulls and the optimal concentration of heparin in the suspension medium in which the highest rate of oocyte penetration took place was 10 mug/ml, indicating that both chemicals act sinergistically to induce capacitation and/or acrosome reaction of spermatozoa and stimulate in vitro fertilization of cattle oocytes.

152 citations




Journal ArticleDOI
TL;DR: The combination of tailp paint, raddling, tailpaint scoring and reraddling is a simple sequence which can be effectively used to detect estrus among heifers synchronized in research or commercial herds.

123 citations


Journal ArticleDOI
TL;DR: Real-time B mode ultrasound systems are available that, if used correctly, can improve the accuracy of pregnancy diagnosis, fetal counts and fetal viability over previous ultrasound systems (A-mode).

119 citations


Journal ArticleDOI
TL;DR: The MGA-PGF(2)alpha system appears to be a better method to synchronize estrus in beef heifers than the SMB system, based on higher synchronized conception and pregnancy rates and lower labor requirements and drug costs.

Journal ArticleDOI
TL;DR: Bovine oocytes were collected from ovaries obtained from an abattoir using a stereomicroscope and cultured in 25 mM Hepes buffered Tissue Culture Medium 199 supplemented with 10% fetal calf serum at 39 degrees C and inseminated by capacitated sperm.

Journal ArticleDOI
K. Bamba1
TL;DR: The highest percentage of non-eosinophilic spermatozoa with an intact acrosome was observed in an eosin-nigrosin solution previously adjusted to physiological pH and osmolality, which was similar to an estimate of acrosomal integrity in frozen-thawed semen determined from smears using differential interference-contrast optics.

Journal ArticleDOI
TL;DR: It is concluded that 30 days after AI, the bPSPB RIA is an efficient test both for pregnancy prediction and detection of nonpregnant dairy cows.

Journal ArticleDOI
TL;DR: The sperm transport efficiency of super-ovulated cows was found to be about 20% lower than those found in single-ovulating cows as mentioned in this paper, which suggests that low fertility of either cows or bulls reduces the fertilization rate, probably through an effect on survival of sperm or efficiency of sperm transport.

Journal ArticleDOI
Barry A. Ball1, S.J. Shin1, V.H. Patten1, D.H. Lein1, G.L. Woods 
TL;DR: Based on microbiologic and cytologic results, the low-volume uterine flush appeared superior to the guarded endometrial swab for the diagnosis of endometritis in mares.

Journal ArticleDOI
TL;DR: A slow cooling rate and a storage temperature of 4 degrees C appear to optimize liquid preservation of equine spermatozoal motility in vitro.

Journal ArticleDOI
TL;DR: Pregnancy rates at Day 35 for mares administered injectable P4 (70%) were identical to those given altrenogest and pregnancy rates for ovariectomized-progestin treated recipients (28 of 40, 70%) were similar to that of intact mares.

Journal ArticleDOI
TL;DR: D diagnostic ultrasonography is a powerful tool for evaluating the reproductive tracts in horses and cattle and should be considered for use in the embryo transfer industry, according to experts.

Journal ArticleDOI
TL;DR: Insemination with high numbers of fresh sperm overcame problems of sperm loss or sperm transport and improved the fertilization rate.

Journal ArticleDOI
TL;DR: Transabdominal real-time ultrasonography is a safe, rapid, accurate and practical method for assessing pregnancy status, fetal number and fetal viability in sheep.

Journal ArticleDOI
TL;DR: The bPSPB RIA detected pregnancy earlier and more accurately than the Pregmatic 3 ultrasonic device and was equally as accurate as the real-time scanning instrument.

Journal ArticleDOI
TL;DR: It is shown that manual removal of RFM can delay the postpartum return to normal reproductive status without altering PGFM profiles.

Journal ArticleDOI
TL;DR: The half-life and the disappearance of pFSH were estimated at 5 h and at 10 to 12 h, respectively, confirming the necessity of using two daily injections to induce superovulation in cows.

Journal ArticleDOI
O. Ohgoda1, Koji Niwa1, M. Yuhara1, S. Takahashi, K. Kanoya 
TL;DR: In this paper, frozen spermatozoa from six different bulls were thawed, washed twice in a caffeine-containing medium and incubated for 5 h at 39°C.

Journal ArticleDOI
TL;DR: The hypothesis that uterine infection may delay the initiation of folliculogenesis and suppress the rate of follicular growth in dairy cows in the immediate postpartum period is supported.

Journal ArticleDOI
TL;DR: Age-constant genetic correlations between yearling body weight and testicular traits were small, indicating that testicular growth and body growth rates are largely independent, regardless of breed.

Journal ArticleDOI
TL;DR: During an attempt to accumulate 40 Bovine Viral Diarrhea virus (BVDV) seronegative cows for breeding and for intramuscular infection on Day 21 of gestation, a persistently infected cow was inadvertently included among the first group of ser onegative animals assembled.

Journal ArticleDOI
TL;DR: The combination of gentamicin (500 ug/ml) tylosin (100 ug /ml) and Linco-Spectin (300/600 ug ) was more effective for the control of mycoplasmas and ureaplasmas than the standard combination of penicillin, dihydrostreptomycin and polymyxin B sulfate.

Journal ArticleDOI
TL;DR: Three experiments were conducted to determine whether coculture of early sheep eggs with oviductal cells would improve the ability of eggs to survive in culture and supported in vitro egg cleavage to a greater degree than did F10FCS alone or F10 FCS with trophoblastic vesicles.