Showing papers in "Zeitschrift für Pflanzenphysiologie in 1982"

TL;DR: Embryoids and plants were obtained from ab initio culture of isolated microspores of Brassica napus (rape) from relatively high concentrations of sucrose in the medium with good yields of embryoids.

TL;DR: An important ecological role for mucilage excretion by root tips in the Al tolerance of plants growing in acid mineral soils is indicated.

TL;DR: In this article, the authors used microelectrodes to measure the pH changes along the roots of sunflower and corn roots in an agar medium containing either bromocresol purple (pH indicator) or BPDS (chelator for FeII).

TL;DR: Continuous darkness used instead of a 16-8 h day-night cycle during both phases favored increase and decrease of peroxidase activity, successively, and induced higher percentages of rooted plantlets.

TL;DR: The rate of appearance of labelled sucrose in the vacuoles closely resembled the rate of sucrose synthesis in the protoplasts and the same was the case for malate and citrate.

TL;DR: It is suggested that thylakoids of mature chloroplasts contain a peroxidase and an oxidase which eventually are activated upon the induction of senescence and become involved in the catabolism of chlorophyll.

TL;DR: A droplet-freezing method has been developed for the cryopreservation of cassava and the meristems retrieved from liquid nitrogen storage exhibited various morphogenetic responses such as differentiation of callus and leaves and whole plantlets.

TL;DR: Cotyledon pieces from mature cassava seeds, cultured on MS basal medium supplemented with various concentrations of 2,4-D and BAP, developed morphogenically-active ridges on their surfaces, which eventually produced both foliose structures and somatic embryos.

TL;DR: Plant regeneration from protoplast derived colonies of B. oleracea, D. innoxia, M. sativa and N. tabacum confirmed the totipotency of cotyledon protoplasts.

TL;DR: Embryos regenerate from salt-tolerant callus cells survived and grew better in saline medium than did embryos regenerated from the original salt sensitive callus, indicating isolation of genetic variants exhibiting stability of the salt-Tolerance trait.

TL;DR: While galactose and lactose over a broad concentration range enhanced embryogenesis in all calli studied, sucrose supported embryogenesis only at the lower levels tested (8–32 mM with various calli).

TL;DR: In this article, the concentration of water-soluble vitamins (thiamine, riboflavin, pyridoxine, niacin, pantothenic acid) and some sugars (glucose, fructose, sucrose, maltose) in the exudates and in the roots of maize seedlings grown for 5 days under sterile conditions in nutrient solution, with or without glass-ballotini, was determined and related to the whole plant, the root dry matter and the dry matter of the total exudate

TL;DR: A limited, but reproducible number of regenerated Brassica napus plants can be obtained via somatic embryos from cultures of isolated protoplasts from stem embryos through a sequence of culture steps including a medium with potato extract.

TL;DR: Water, osmotic and pressure potentials of developing embryos and related structures of Phaseolus vulgaris var.

TL;DR: In this paper, the photosynthetic apparatus of spinach (Spinacia oleracea L. var. Matador) grown in sandy soil containing excess amounts of copper was investigated.

TL;DR: Mesophyll and cell suspension protoplasts of Medicago coerulea and M. glutinosa divided to form callus and the potential of protoplast systems for transfer of characteristics from M. coerceda to M. sativa is discussed.

TL;DR: A general method for rapidly producing callus from the roots of germinating oat seedlings is discussed and the occurrence of green spots in secondary callus is positively correlated with shoot regeneration although only regeneration decreases with time.

TL;DR: By rigorously adhereing to the method detailed in this paper, an average of one hundred Lukullus plants can be regenerated from one million protoplasts.

TL;DR: The distribution of metals in algae exposed to the metals in cultures under laboratory conditions was similar to that in algae growing at sites with high concentrations of heavy metals in the sea water.

TL;DR: A comparison of previously published data on halophytes with similar data for D. spicata suggests that a considerable proportion of the salt tolerance expressed by this halophytic monocot is cellularly based and is not dependent upon anatomical and physiological specializations of whole plants.

TL;DR: Protoplasts were isolated from seedling roots of Medicago sativa and Trigonella foenumgraecum using a simple enzyme mixture and plantlets were recovered via somatic embryogenesis from protoplast derived tissues.

TL;DR: The use of embryogenic callus offers a significant advance in the control of regeneration in oats and a model which explains the ontological relationship between observed calli types and green spots is given.

TL;DR: In this article, it was shown that increasing the amount of nitrogen added to the pre-cultured plants by increasing the rate of nitrogen addition was the decisive factor regulating the growth of the plants.

TL;DR: Protoplasts of lettuce, Lactuca sativa L. sativa can be obtained from greenhouse and growth chamber grown plants, in vitro grown shoots and roots and cotyledons of seedlings and were most readily obtained with protoplasts from seedling plant material.

TL;DR: Large populations of protoplasts from five species: Euphorbia lathyris, Nicotiana glauca, N. langsdorfii, Petunia parodii and P. inflata have been characterized by flow cytometry on the basis of laser light scatter and chlorophyll fluorescence or exogenously-added stains, fluorescein isothiocyanate (FITC) and rhodamine isothionate (RITC).

TL;DR: The hairy root regenerants show the same overall morphological characteristics of normal plants and are susceptible to infection with both Agrobacterium rhizogenes and Agrobacteria tumefaciens, but their flowers are all characteristically modified in that they show pistils much longer than the stamens (heterostyly).

TL;DR: Changes in cytokinin content of developing wheat grains were investigated beginning at ear emergence and were maximal 4 d after anthesis although the glucosides showed increased levels at an earlier developmental stage than either zeatin orZeatin riboside.

TL;DR: Frost resistance of spruce needles is regulated by the environmental factors temperature and photoperiod and the regulation of the metabolism of membrane lipids on the basis of phytohormone balance is discussed.

TL;DR: Replacement of ammonium and nitrate by glutamine as the sole nitrogen source improved plating efficiency and cell colony survival and plant regeneration from protoplast-derived calli was achieved through shoot formation.

TL;DR: In this paper, the polar lipids of two subsequent spruce needle generations were investigated during the course of one year, and a maximum lipid content, as calculated on a chlorophyll basis, occurred in winter when the needles were frost resistant down to temperatures of -30 °C and lower.