Journal ArticleDOI
Plant Regeneration from Seedling Cotyledon Protoplasts
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TLDR
Plant regeneration from protoplast derived colonies of B. oleracea, D. innoxia, M. sativa and N. tabacum confirmed the totipotency of cotyledon protoplasts.About:
This article is published in Zeitschrift für Pflanzenphysiologie.The article was published on 1982-07-01. It has received 80 citations till now. The article focuses on the topics: Cotyledon & Nicotiana tabacum.read more
Citations
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Journal ArticleDOI
High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae
TL;DR: To obtain a high frequency of regeneration, rapidly growing calli were transferred to media having a high cytokinin:auxin ratio as early as possible, usually 3 weeks after protoplast isolation.
Journal ArticleDOI
Plant regeneration from suspension culture and mesophyll protoplasts of Medicago sativa L.
TL;DR: A system was established for achieving plant regeneration from mesophyll protoplasts and cotyledon-derived cell suspension cultures of alfalfa, Medicago sativa L. Citation, Answer and Regen S, the latter two produced embryos from which plants could be regenerated.
Book ChapterDOI
Somatic Embryogenesis in Herbaceous Dicots
TL;DR: The seed is the primary organ for the perpetuation of germplasm and propagation in all flowering plants and contains the embryo which develops from the zygote following the fertilization of the egg cell by one of the male gametes.
Book
Legumes and Oilseed Crops I
TL;DR: This chapter discusses wide Hybridization in Legumes and Oilseed Crops Through Embryo, Ovule, and Ovary Culture, as well as methods for Isolation, Culture, and Electrofusion of Glycine Protoplasts.
Journal ArticleDOI
A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus.
TL;DR: Protoplasts of several spring and winter varieties of Brassica napus were isolated from hypocotyl tissue and divided and formed cell colonies at high frequency, without browning when cultured in modified Shepards' medium.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
The isolation, culture and regeneration of Petunia leaf protoplasts.
TL;DR: Protoplasts isolated from varieties of P. hybrida were found to differ in their cultural requirements, and cell colonies derived from individual mesophyll protoplasts grow rapidly upon subculture, to produce callus capable of shoot differentiation and ultimately whole plant formation.
Journal ArticleDOI
Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca
TL;DR: Protoplasts of soybean and N. glauca were induced to fuse with polyethylene glycol (PEG 1540) and up to 39% of the protoplasts in the treated population were heterokaryocytes, which indicated a tendency to stick together and break into pieces in the later cell generations.
Journal ArticleDOI
Mesophyll Cell Protoplasts of Potato: Isolation, Proliferation, and Plant Regeneration
James F. Shepard,Roger E. Totten +1 more
TL;DR: Shooting bud development within p-calli was controlled by a number of factors including light, temperature, basic medium composition, nature and source of phytohormones, the continued presence of an osmoticum, low concentrations of a utilizable carbohydrate, and the developmental stage of the p- callus.
Journal ArticleDOI
Plant Regeneration from Mesophyll Protoplasts of Alfalfa
K.N. Kao,M.R. Michayluk +1 more
TL;DR: Embryoids were initiated from alfalfa mesophyll protoplasts and subsequently regenerated to plants and the ability of these protoplast to form embryoids and regenerate into plants varied considerably from plant to plant within the same cultivar.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
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High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae
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