Journal ArticleDOI
4S polycyclic aromatic hydrocarbon receptor (glycine N-methyltransferase) and the aryl hydrocarbon receptor nuclear translocator (hypoxia inducible factor-1β) interaction in Chinese hamster ovary and rat hepatoma cells: 4S PAH-R/ARNT hetero-oligomers?
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Results of this analysis indicated that the 4S PAH receptor/GNMT forms a hetero‐oligomer (dimer?) with ARNT/HIF‐1β which dissociates in the presence of B[a]P, indicating the role of GNMT (which has been shown to be multifunctional) and B[ a]P in the induction of CYP1A1 and also a potential role in the modulation of hypoxia inducible factor‐Abstract:
Rat CYP1A1 promoter-luciferase, transiently transfected wild-type and 4S PAH receptor (glycine N-methyl transferase, GNMT)-transformed Chinese hamster ovary (CHO) cells were exposed to benzo[a]pyrene and assayed for luciferase activity as an indicator of CYP1A1 promoter activity. CHO cells transformed with the rat 4S PAH receptor/GNMT expression vector had twice the induction level of luciferase activity with respect to wild-type CHO cells in concert with previously published reports that the 4S PAH receptor/GNMT mediates benzo[a]pyrene induction of CYP1A1 gene expression. Lysates of GNMT-transformed CHO cells and wild-type H4IIE rat hepatoma cells exposed to benzo[a]pyrene were immuno-precipitated with anti-GNMT antibodies, separated by SDS-polyacrylamide gel electrophoresis and transferred to PVDF membrane for Western blot analysis with anti-aryl hydrocarbon receptor nuclear translocator (ARNT, HIF-1β) antibodies. Results of this analysis indicated that the 4S PAH receptor/GNMT forms a hetero-oligomer (dimer?) with ARNT/HIF-1β which dissociates in the presence of B[a]P. These observations further indicate the role of GNMT (which has been shown to be multifunctional) and B[a]P in the induction of CYP1A1 and also a potential role of GNMT in the modulation of hypoxia inducible factor-1 function with respect to the HIF-1β subunit (ARNT).read more
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Mammalian Sulfur Amino Acid Metabolism: A Nexus Between Redox Regulation, Nutrition, Epigenetics, and Detoxification
TL;DR: Increasing the knowledge on transsulfuration outside the liver, understanding the protein-protein interaction networks involving these enzymes, the functional role of their PTMs, or the mechanisms controlling their nucleocytoplasmic shuttling may provide further insights into the pathophysiological implications of this pathway, allowing design of new therapeutic interventions.
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Glucoraphanin does not reduce plasma homocysteine in rats with sufficient Se supply via the induction of liver ARE-regulated glutathione biosynthesis enzymes
Nicole M. Blum,Kristin Mueller,Frank Hirche,Doris Lippmann,Erika Most,Josef Pallauf,Thomas Linn,Andreas S. Mueller +7 more
TL;DR: The hypothesis that GRA supplementation to rat diets lowers plasma HCys levels by increasing GSH synthesis is tested and it is concluded that the long-term supplementation of moderate GRA doses reduces ARE-driven gene expression in the liver by increasing the intestinal barrier against oxidative stress.
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Nuclear localization dictates hepatocarcinogenesis suppression by glycine N-methyltransferase
TL;DR: In this paper , the authors investigated the oncosuppressive mechanisms of GNMT in human hepatocellular carcinoma (HCC) and found that GNMT forced expression inhibited the proliferation and promoted apoptosis.
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Nuclear localization dictates hepatocarcinogenesis suppression by glycine N-methyltransferase.
Maria Maddalena Simile,Antonio Cigliano,Panagiotis Paliogiannis,Lucia Daino,Roberto Manetti,Claudio F. Feo,Diego F. Calvisi,Francesco Feo,Rosa Maria Pascale +8 more
TL;DR: In this paper, the authors investigated the oncosuppressive mechanisms of GNMT in human hepatocellular carcinoma (HCC) and found that GNMT forced expression inhibited the proliferation and promoted apoptosis.
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Chronic Kidney Disease Induces Proarrhythmic Remodeling
TL;DR: In this article , the authors examined the relationship between kidney disease progression and structural, electrophysiological, and molecular cardiac remodeling, and found that female sex is a protective factor in the progression of CKD and its cardiac sequelae.
References
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Journal Article
Pharmacological implications of microsomal enzyme induction
TL;DR: It is of considerable interest that certain inducers of liver microsomal enzymes have recently been used therapeutically for the treatment of hyperbilirubinemia in jaundiced children and for thetreatment of Cushing's syndrome.
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Induction of Microsomal Enzymes by Foreign Chemicals and Carcinogenesis by Polycyclic Aromatic Hydrocarbons: G. H. A. Clowes Memorial Lecture
TL;DR: An important problem that has been of great interest to me for many years is individuality in the response of human beings and other living organisms to foreign chemicals.
Journal ArticleDOI
P450 genes: structure, evolution, and regulation
Journal ArticleDOI
The PAS Superfamily: Sensors of Environmental and Developmental Signals
TL;DR: This review provides a detailed description of three signal transduction pathways that utilize PAS protein heterodimers to drive their transcriptional output and develops the idea that most eukaryotic PAS proteins can be classified by functional similarities, as well as by predicted phylogenetic relationships.
Journal ArticleDOI
Cloning of a factor required for activity of the Ah (dioxin) receptor.
E.C. Hoffman,Herminio Reyes,Fong-Fong Chu,Fred C. Sander,Linda H. Conley,B.A. Brooks,Oliver Hankinson +6 more
TL;DR: The complementary DNA and part of the gene for an 87-kilodalton human protein that is necessary for Ah receptor function have been cloned and two portions of the protein share sequence similarities with two Drosophila proteins, Per and Sim.