Proceedings ArticleDOI
7,12-dimethylbenz(a)anthracene induced breast cancer in Sprague Dawley rats proved by ultrasound imaging
Mutmainnah Arif,Padlianah,Ika Yustisia +2 more
- Vol. 2108, Iss: 1, pp 020040
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TLDR
This preliminary research aimed to develop rat breast cancer models that will be used for a further study on phytotherapy as the alternative medicine for breast cancer.Abstract:
Many studies have shown that some of cancer animal models have been established to study various aspects of cancer biology, including breast cancer. Substantial evidence has shown that administration of 7,12-dimethylbenz(a)anthracene (DMBA) in animal models can mimic human breast cancer in many ways. This preliminary research aimed to develop rat breast cancer models that will be used for a further study on phytotherapy as the alternative medicine for breast cancer. Four young Sprague Dawley female rats, aged 60 days, received a single subcutaneous injection of 25 mg DMBA mixed with 0.5 mL sunflower oil and 0.5 mL sodium chloride 0.9%. Control group received 0.5 ml of sunflower oil and 0.5 ml of saline only. Tumor detection by palpation and rats’ body weight measurement were performed every week after DMBA induction. At 10-weeks after DMBA induction, the tumor development was examined by ultrasound imaging. No significant differences were observed in the body weight between DMBA-induced rats and non-induced rats during 8-weeks after induction. Starting from week ninth, the body weight of the DMBA group significantly decreased (p=0.02) compared to the counterpart. A solid mass on the breast area was firstly detected at week seventh in one rat. Three weeks later the solid masses were detected in all DMBA-induced rats that proved by the ultrasound images showing hypoechoic lesions with irregular margin and calcification indicating malignancies. DMBA induced malignant lesions in female rats that demonstrated by ultrasound imaging.read more
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Journal ArticleDOI
Novel doxorubicin / folate-targeted trans-ferulic acid-loaded PLGA nanoparticles combination: In-vivo superiority over standard chemotherapeutic regimen for breast cancer treatment.
TL;DR: In this paper, the authors investigated the antitumor efficacy of Doxorubicin (Dox)/FA-PLGA-TFA NPs combination against DMBA-induced breast cancer and its safety profile.
Journal ArticleDOI
Novel doxorubicin / folate-targeted trans-ferulic acid-loaded PLGA nanoparticles combination: In-vivo superiority over standard chemotherapeutic regimen for breast cancer treatment
TL;DR: In this paper , the authors investigated the antitumor efficacy of Doxorubicin (Dox)/FA-PLGA-TFA NPs combination against DMBA-induced breast cancer and its safety profile.
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Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study
Harneetpal Kaur,Kirandeep Kaur,Atamjit Singh,Neena Bedi,Balbir Singh,Mansour Alturki,Mohammed F. Aldawsari,Atiah H. Almalki,Shafiul Haque,Hae-Jeung Lee,Dharmendra Kumar Yadav,Saroj Arora +11 more
TL;DR: Optimized nanoemulsion of paclitaxel and erucin combination (EPNE) provided sustained release and exhibited enhanced cytotoxicity towards human epithelial breast cancer cells (T-47D) as compared to individual ER and PTX.
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Tenofovir alone or combined with doxorubicin abrogates DMBA-induced mammary cell carcinoma: An insight into its modulatory impact on oxidative/Notch/apoptotic signaling.
TL;DR: In this paper , the antiviral tenofovir disproxil fumarate (TF) was reported to decrease hepatocellular carcinoma risk by interfering with cell cycle and proliferation.
References
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Book ChapterDOI
Superoxide dismutase assays.
TL;DR: This chapter presents a qualitative test for SOD activity based on the reduction of nitro blue tetrazolium (NBT) by O 2 · -, a simple immunological determination of the SOD molecule of sufficient sensitivity and avoiding labeled reagents, and an indirect measure of Sod activity to be used in purified samples.
Journal ArticleDOI
Carcinogen-specific mutation and amplification of Ha- ras during mouse skin carcinogenesis
TL;DR: A specific A→T transversion at the second nucleotide of codon 61 of the Harvey-ras (Ha-ras) gene is found, suggesting that the mutation occurs at the time of initiation of tumour progression.
Journal ArticleDOI
Pemanfaatan Obat Tradisional Dengan Pertimbangan Manfaat Dan Keamanannya
TL;DR: Side effects of herbal medicines can be reduced with the used of right materials, accuracy of materials, dose, USAge time, accurat way of USAge, analyze information, and without abusing of herbal medicine itself.
Journal ArticleDOI
Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene
Alfredo Carlos Simões Dornellas de Barros,Elisa Naomi K. Muranaka,Lincon Jo Mori,Christina Helena T. Pelizon,Kyoshi Iriya,Giovana Giocondo,José Aristodemo Pinotti +6 more
TL;DR: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.
Journal ArticleDOI
The chromosomes of primary 7,12‐dimethylbenz(a)anthracene‐induced rat sarcomas
Felix Mitelman,Göran Levan +1 more
TL;DR: The chromosomes of 12 primary 7,12-dimethylbenz(a)anthracene-induced rat sarcomas were studied in direct fixations and indicate that each oncogenic agent induces specific karyotypic changes.
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