Showing papers in "Biophysical Journal in 1987"

TL;DR: The probability density function (pdf) corresponding to logarithmically binned histograms is derived, a peaked function with an invariant width, coupled with a variance-stabilizing transformation for the ordinate, to greatly simplify the interpretation and manual fitting of distributions containing multiple exponential components.

TL;DR: MEM could be a powerful tool to analyze the heterogeneity of fluorescent emission of biological macromolecules which can be correlated with their conformational dynamics in solution.

TL;DR: The response of the model to repetitive stimulation allows comprehension, in terms of receptor desensitization, of the role of periodic signaling in Dictyostelium and, more generally, the function of pulsatile patterns of hormone secretion.

TL;DR: An approach based on protein dynamics is presented, which provides fluorescence lifetime distribution functions for single tryptophan residue proteins and the origin of negative components of the lifetime distribution is discussed.

TL;DR: The temperature dependence of the parameters that describe the single-potential well distribution indicated the inadequacy of this model at lower temperature, suggesting that multiple potential wells can describe better the decay for this protein.

TL;DR: A physical approach is proposed, which provides lifetime distribution functions for the tryptophan decay in proteins, and it is shown that real lifetime distributions can be proved using multimodal probability density functions.

TL;DR: A dynamical model for receptor-mediated adhesion of cells in a shear field of viscous fluid to surfaces coated with ligand molecules complementary to receptors in the cell membrane is presented.

TL;DR: Simulations of the residual configurational entropy of a protein in the native state suggest that it is nearly an order of magnitude larger than the entropy of denaturation.

TL;DR: Monte Carlo calculations of diffusion constants on square and triangular lattices as a function of the concentration of obstacles and the relative jump rate are presented and the concentration-dependent diffusion constant is obtained.

TL;DR: Results of parameter estimation for intracellular particle behavior both within control cells and after variable magnetization duration are presented, which showed cytoplasmic elasticity, dose-dependent motion inhibition by cytochalasin D, and a shear-thinning apparent viscosity.

TL;DR: Methods are described for discrimination of models of the gating kinetics and permeation of single ionic channels, including Monte Carlo methods, which allow the comparison of models at significance levels of choice.

TL;DR: The physical principles relevant to magnetometry are presented and models for intracellular particle motion driven by thermal, elastic, or cellular forces are suggested and described.

TL;DR: Frog gastrocnemius muscles stimulated isometrically in a saline bath at 20 degrees C were found to produce a single ringing sound event beginning just before the tension record began to rise, consistent with the conclusion that the sounds in these muscles are caused primarily by transverse resonant vibrations.

TL;DR: The kinetics of ion channels were found to be statistically self-similar at different time scales as predicted by the fractal model, and the open and closed times and the voltage dependence of the gating of this channel are well described.

TL;DR: A new automatic technique based on the visualization of colloidal gold particles by video-enhanced contrast light microscopy (nanometer video microscopy) combined with modern tracking algorithms and image processing hardware is described, which can be used for determining the complete statistics of saltatory motility of a large number of individual moving markers.

TL;DR: Results provided evidence that sound production is produced by lateral oscillations of muscle, and the oscillation frequency may provide a measure of mechanical properties of muscle.

TL;DR: The use of high order autocorrelation in fluorescence correlation spectroscopy for investigating aggregation in a sample that contains fluorescent molecules is described and may prove useful in detecting and characterizing aggregation of fluorescent-labeled biological molecules such as cell surface receptors.

TL;DR: It is concluded that the crypt and villus populations in the adult mouse are not in a steady state and a crypt cycle (analogous to the cell cycle) is proposed and evidence that most if not all crypts in theAdult mouse are cycling asynchronously and independently is presented.

TL;DR: Monolayers of the phospholipid dimyristoyl phosphatidic acid on the surface of water have been studied by a combination of the new techniques of synchrotron x-ray diffraction and fluorescence microscopy with classical surface pressure data to demonstrate that between pi c and pi s the fluid phase coexists with a denser "gel" phase.

TL;DR: A new constitutive formulation that combines certain desirable features of two previously used approaches (phenomenological and microstructural) is presented, wherein load-dependent anisotropy, nonlinear material behavior, finite deformations, and incompressibility are accounted for.

TL;DR: The large number of observed bands suggests that slight variations in helical geometry, symmetry, or interactions result in changed amide III frequencies, so that simple correlations between narrow frequency ranges and secondary structures may not be applicable for this mode.

TL;DR: A general procedure for using myoplasmic calcium transients measured with a metallochromic indicator dye to calculate the time course of calcium release from the sarcoplasmics reticulum in voltage-clamped skeletal muscle fibers is described and analyzed.

TL;DR: It is suggested that the two signals originate from separate PS II electron donors that are in a redox equilibrium with each other in the S(2) state and that the g = 4.1 signal arises from monomeric Mn(IV).

TL;DR: Application of the analysis to precise measurements of the relationship between tether radius and axial force will provide a novel method for determining the mechanical properties of biomembrane.

TL;DR: The reaction of fluorescence-labeled alamethicin with unilamellar phospholipid vesicles (DOPC and DMPC) has been investigated in a stopped-flow apparatus and the pseudo first-order forward rate is found to be quite fast, falling in a range somewhat below the diffusion controlled upper bound.

TL;DR: Voltage clamped and internally perfused freshly isolated canine cardiac Purkinje cells using a large bore (25-microns diam) double-barreled flow-through glass suction pipette yielded good conditions for the study of excitatory sodium current (INa).

TL;DR: Large unitary conductance Ca2+-activated K+ channels from smooth muscle membrane were incorporated into phospholipid planar bilayers, and the blockade induced by internally and externally applied Cs+ was characterized, suggesting that the channel behaves as a multi-ion pore.

TL;DR: The results suggest that the major effect of filament lattice spacing modulates contractile function is upon the rate constant for cross-bridge detachment.

TL;DR: It is shown that the fluorescence kinetics of C-Phycocyanin is expected to be characterized by three exponential kinetic components, independent of the aggregation state, which allows for a detailed understanding of the origin of the different kinetic components and their associated time-resolved spectra.

TL;DR: Computer-assisted analysis of stroboscopic photomicrographs of live sea urchin spermatozoa whose creatine kinase has been specifically inhibited by fluorodinitrobenzene reveals that motility is impaired due to a progressive damping of bending waves as they propagate along the flagellum.