Journal ArticleDOI
A lactate electrode with lactate oxidase immobilized on nylon net for blood serum samples in flow systems
TLDR
Commercially available lactate oxidase from Mycobacterium smegmatis is immobilized on a nylon net which is fixed on an oxygen probe to provide a simple l -lactate sensor and effects of pH, temperature and different buffers are described.About:
This article is published in Analytica Chimica Acta.The article was published on 1984-01-01. It has received 83 citations till now. The article focuses on the topics: Enzyme electrode & Blood serum.read more
Citations
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Polymers in sensor applications
TL;DR: The role of polymers as gas sensors, pH sensors, ion-selective sensors, humidity sensors, biosensor devices, etc., are reviewed and discussed in this article, and current trends in sensor research and also challenges in future sensor research are discussed.
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Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole–polyvinylsulphonate composite films for application to lactate biosensors
TL;DR: In this article, the immobilization of lactate dehydrogenase (LDH) on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPY-PVS) films has been accomplished via cross-linking technique using glutaraldehyde.
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Amperometric lactate biosensors and their application in (sports) medicine, for life quality and wellbeing
Nadia Nikolaus,Beate Strehlitz +1 more
TL;DR: The article reviews the use of electrochemical biosensors for detecting lactate, a key metabolite of the anaerobic glycolytic pathway, which plays an important role in (sports) medicine, in the nutritional sector, in food quality control and touches environmental concerns.
Journal ArticleDOI
Dynamic Electrochemistry: Methodology and Application
TL;DR: Revue bibliographique de 1983 a 1985 classee suivant: livres, transfert de masse, detecteurs, cinetique, effets de surface, electrodes modifiees chimiquement, voltammetrie analytique, caracterisation des systemes redox, bioelectrochimie, spectroelectro Chimie.
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An enzyme electrode forl-lactate with a chemically-amplified response
TL;DR: In this paper, an enzyme electrode with a chemically-amplified response for l -lactate is constructed from an oxygen electrode and a layer containing immobilized lactate oxidase, to oxidize l - lactate, and lactate dehydrogenase to regenerate the l − lactic acid.
References
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Journal ArticleDOI
Mechanism of action of the flavoenzyme lactate oxidase.
TL;DR: The intermediate is a charge transfer complex of reduced enzyme and keto acid and that it participates in catalysis, and is concluded that the pathway for acetate production includes the nonenzymatic reaction of these two substances.
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Enzyme electrodes with improved mechanical and analytical characteristics obtained by binding enzymes to nylon nets
TL;DR: In this article, the chemical binding process is mild so that the final membrane retains a high enzyme activity and the resulting membrane also has high mechanical strength and can be assembled on the electrode surface several times without damage.
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Sequential determination of L-lactate and lactate dehydrogenase with immobilized enzyme electrode
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Mechanism of inactivation of the flavoenzyme lactate oxidase by oxalate.
Sandro Ghisla,Vincent Massey +1 more
TL;DR: Lactate oxidase from Mycobacterium smegmatic is strongly inhibited by oxalate, and its binding is described by a single step reversible process, which is second order in oxamate.