A Novel Approach to Improving the Reliability of Manual Semen Analysis: A Paradigm Shift in the Workup of Infertile Men
Christopher Douglas,Neel Parekh,Linda G. Kahn,Linda G. Kahn,Ralf Henkel,Ralf Henkel,Ashok Agarwal +6 more
TLDR
Assessing ORP is a novel approach to both validating manual SA results and identifying patients who may benefit from treatment of male oxidative stress infertility.Abstract:
Conventional semen analysis (SA) is an essential component of the male infertility workup, but requires laboratories to rigorously train and monitor technicians as well as regularly perform quality assurance assessments. Without such measures there is room for error and, consequently, unreliable results. Furthermore, clinicians often rely heavily on SA results when making diagnostic and treatment decisions, however conventional SA is only a surrogate marker of male fecundity and does not guarantee fertility. Considering these challenges, the last several decades have seen the development of many advances in SA methodology, including tests for sperm DNA fragmentation, acrosome reaction, and capacitation. While these new diagnostic tests have improved the scope of information available to clinicians, they are expensive, time-consuming, and require specialized training. The latest advance in laboratory diagnostics is the measurement of seminal oxidation-reduction potential (ORP). The measurement of ORP in an easy, reproducible manner using a new tool called the Male Infertility Oxidative Stress System (MiOXSYS) has demonstrated ORP's potential as a feasible adjunct test to conventional SA. Additionally, the measurement of ORP by this device has been shown to be predictive of both poor semen quality and male infertility. Assessing ORP is a novel approach to both validating manual SA results and identifying patients who may benefit from treatment of male oxidative stress infertility.read more
Citations
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TUNEL assay-Standardized method for testing sperm DNA fragmentation.
TL;DR: The protocol, quality control and measurement of sperm DNA fragmentation using a benchtop flow cytometer using the terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate‐nick end labelling TUNEL assay using flow cytometry is described.
Journal ArticleDOI
Transcriptomic Profiling of Buffalo Spermatozoa Reveals Dysregulation of Functionally Relevant mRNAs in Low-Fertile Bulls.
Nilendu Paul,Arumugam Kumaresan,Mohua Das Gupta,Pradeep Nag,Pushpa Rani Guvvala,Channareddi Kuntareddi,Ankur Sharma,Sellappan Selvaraju,Tirtha Kumar Datta +8 more
TL;DR: In this paper, the differences in sperm functional attributes and transcriptomic profile between high- and low-fertile buffalo bulls were compared and the results of the study open up new avenues for understanding the etiology for poor fertility in buffalo bulls and to identify fertility biomarkers.
Journal ArticleDOI
Evaluation of seminal Oxidation–Reduction potential in male infertility
TL;DR: Oxidation–reduction potential (ORP) or redox potential, is a measure of the electron transfer from antioxidants to oxidants and provides information on the redox balance and its importance in the context of male infertility is discussed.
Journal ArticleDOI
Microenvironment for spermatogenesis and sperm maturation
TL;DR: Three factors, including genetic deletion or abnormalities, aberration of DNA methylation and histone modifications, as well as small RNA dysfunction, and neuroendocrine-immune disorders are involved in functional failure of the male reproductive system are reviewed.
Journal ArticleDOI
Standardized Laboratory Procedures, Quality Control and Quality Assurance Are Key Requirements for Accurate Semen Analysis in the Evaluation of Infertile Male
Ashok Agarwal,Rakesh Sharma,Sajal Gupta,Renata Finelli,Neel Parekh,Manesh Kumar Panner Selvam,Camila Pompeu,Sarah Madani,Andrea Belo,Mahsa Darbandi,Neha Singh,Sara Darbandi,Simryn Covarrubias,Raha Sadeghi,Mohamed Arafa,Mohamed Arafa,Ahmad Majzoub,Ahmad Majzoub,Meaghanne Caraballo,Alyssa Giroski,Kourtney McNulty,Damayanthi Durairajanayagam,Ralf Henkel,Ralf Henkel +23 more
TL;DR: In this article, a narrative review aims to describe standardized laboratory procedures for an accurate assessment of semen parameters that incorporate both quality control (QC) and quality assurance (QA) practices.
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