A Universal Chemical Method for Rational Design of Protein-Based Nanoreactors*.
TLDR
In this paper, Micelle-assisted protein labeling (MAPLab) technology along with the N-terminal bioconjugation strategy is utilized for the synthesis of a well-defined monodisperse self-assembling semi-synthetic protease.Abstract:
Self-assembly of a monomeric protease to form a multi-subunit protein complex "proteasome" enables targeted protein degradation in living cells. Naturally occurring proteasomes serve as an inspiration and blueprint for the design of artificial protein-based nanoreactors. Here we disclose a general chemical strategy for the design of proteasome-like nanoreactors. Micelle-assisted protein labeling (MAPLab) technology along with the N-terminal bioconjugation strategy is utilized for the synthesis of a well-defined monodisperse self-assembling semi-synthetic protease. The designed protein is programmed to self-assemble into a proteasome-like nanostructure which preserves the functional properties of native protease.read more
Citations
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Posted ContentDOI
Rational Design of Self-assembling Artificial Proteins Utilizing a Micelle-Assisted Protein Labeling Technology (MAPLabTech): Testing the Scope
TL;DR: In this article, the synthesis of self-assembling artificial proteins (SAPs) using micelle-assisted protein labeling technology (MAPLabTech) has been described by developing a chemical method for site-specific labeling of a surfaceexposed cysteine residue of globular proteins.
Journal ArticleDOI
Rational Design of Self‐Assembling Artificial Proteins Utilizing a Micelle‐Assisted Protein Labeling Technology (MAPLabTech): Testing the Scope **
Mullapudi Mohan Reddy,Pavankumar Janardhan Bhandari,Kshitish Chandra Hati,Britto S. Sandanaraj +3 more
TL;DR: The synthesis of novel SAPs are described by developing a chemical method for site‐specific labeling of a surface‐exposed cysteine residue of globular proteins and their systematic self‐assembly and disassembly studies using size‐exclusion chromatography are disclosed.
References
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Journal ArticleDOI
Protein Assembly: Versatile Approaches to Construct Highly Ordered Nanostructures
TL;DR: This Review outlines recent advances in the field of protein assembly and summarizes several strategies, including biotechnological strategies, chemical strategies, and combinations of these approaches, for manipulating proteins to self-assemble into desired nanostructures.