Active recruitment of σ54‐RNA polymerase to the Pu promoter of Pseudomonas putida: role of IHF and αCTD
TLDR
The view that binding of σ54‐RNAP to a promoter is a step that can be subjected to regulation by factors (e.g. IHF) other than the sole intrinsic affinity of ρ54‐ RNAP for the −12/−24 site is supported.Abstract:
The sequence elements determining the binding of the sigma54-containing RNA polymerase (sigma54-RNAP) to the Pu promoter of Pseudomonas putida have been examined. Contrary to previous results in related systems, we show that the integration host factor (IHF) binding stimulates the recruitment of the enzyme to the -12/-24 sequence motifs. Such a recruitment, which is fully independent of the activator of the system, XylR, requires the interaction of the C-terminal domain of the alpha subunit of RNAP with specific DNA sequences upstream of the IHF site which are reminiscent of the UP elements in sigma70 promoters. Our data show that this interaction is mainly brought about by the distinct geometry of the promoter region caused by IHF binding and the ensuing DNA bending. These results support the view that binding of sigma54-RNAP to a promoter is a step that can be subjected to regulation by factors (e.g. IHF) other than the sole intrinsic affinity of sigma54-RNAP for the -12/-24 site.read more
Citations
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Carbon catabolite repression in Pseudomonas : optimizing metabolic versatility and interactions with the environment
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Promoters in the environment: transcriptional regulation in its natural context
TL;DR: This work has shown that in vivo transcription initiation of bacterial promoters must process various physicochemical and metabolic signals to determine their output, which helps to achieve optimal bacterial fitness in extremely competitive niches.
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