Proceedings ArticleDOI
Adaptive aberration correction for specimen induced spherical aberration
L. Sherman,Jing Yong Ye,Theodore B. Norris +2 more
- pp 330-331
TLDR
A genetic learning algorithm is applied in conjunction with third harmonic generation to determine the appropriate shape of the deformable mirror to correct for aberrations in longitudinal depth scanning in a confocal microscope.Abstract:
Summary form only given. In normal operation with a dry or oil immersion microscope objective there is an index mismatch with a water-based biological sample. This index mismatch causes a depth dependant spherical aberration that severely limits the range that a sample can be imaged along the optical axis. We have previously demonstrated the use of a membrane deformable mirror to correct for off-axis aberrations in a confocal microscopy system. In this study we apply a genetic learning algorithm in conjunction with third harmonic generation to determine the appropriate shape of the deformable mirror to correct for aberrations in longitudinal depth scanning in a confocal microscope.read more
References
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Journal ArticleDOI
Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: an integral representation
TL;DR: In this paper, the diffraction problem for a planar interface between two isotropic and homogeneous materials with this interface perpendicular to the optical axis is solved in a rigorous mathematical manner, and it satisfies the homogeneous wave equation.
Journal ArticleDOI
Smart microscope: an adaptive optics learning system for aberration correction in multiphoton confocal microscopy.
TL;DR: This adaptive optical correction scheme is implemented in an all-reflective system by use of extremely short (10-fs) optical pulses, and it is shown that the scanning area of an f:1 off-axis parabola can be increased by nine times with this technique.
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