Proceedings ArticleDOI
Adaptive optics in microscopy
TLDR
Applications of adaptive optics in the related areas of optical data storage, optical tweezers and micro/nanofabrication are reviewed, particularly in confocal and two-photon microscopes.Abstract:
Confocal microscopes unlike their conventional counterparts have the ability to optically ‘section’ thick specimens. However the resolution and optical sectioning can be severely degraded by system or specimen-induced aberrations. The use of high aperture lenses further exacerbates the difficulties. We will describe an adaptive optics solution to this fundamental problem.read more
Citations
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Journal ArticleDOI
Clarifying Tissue Clearing.
TL;DR: The physical basis for light scatter in tissue is introduced, the mechanisms underlying various clearing techniques are described, and several of the major advances in light microscopy for imaging cleared tissue are discussed.
Journal ArticleDOI
Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues
TL;DR: An approach to adaptive optics in microscopy wherein the rear pupil of an objective lens is segmented into subregions, and light is directed individually to each subregion to measure, by image shift, the deflection faced by each group of rays as they emerge from the objective and travel through the specimen toward the focus.
Journal ArticleDOI
Adaptive optical microscopy: the ongoing quest for a perfect image
Martin J. Booth,Martin J. Booth +1 more
TL;DR: How technologies such as deformable mirrors and spatial light modulators, which compensate for aberrations by locally controlling the wavefront of a light wave, are now improving the performance of multiphoton, confocal, widefield and super-resolution microscopes are reviewed.
Journal ArticleDOI
Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking
TL;DR: In this article, a variety of methods for obtaining both 3D super-resolution images and 3D tracking infers structures or motions extending in the axial direction can easily be missed or confused.
Journal ArticleDOI
Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens
Liang Gao,Lin Shao,Christopher D. Higgins,John S. Poulton,Mark Peifer,Michael W. Davidson,Xufeng Wu,Bob Goldstein,Eric Betzig +8 more
TL;DR: It is reported that rapid three-dimensional dynamics can be studied beyond the diffraction limit in thick or densely fluorescent living specimens over many time points by combining ultrathin planar illumination produced by scanned Bessel beams with super-resolution structured illumination microscopy.
References
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Journal ArticleDOI
A revolution in optical manipulation
TL;DR: This research presents the next generation of single-beam optical traps, which promise to take optical tweezers out of the laboratory and into the mainstream of manufacturing and diagnostics and even become consumer products.
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
TL;DR: In this paper, a multiphoton microscopy based on coherent anti-Stokes Raman scattering with near-infrared ultrashort laser pulses is demonstrated for chemical and biological samples with high sensitivity, high spatial resolution, noninvasiveness, and three-dimensional sectioning capability.
Journal Article
Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
TL;DR: In this article, a CARS-based multiphoton microscopy based on coherent anti-Stokes Raman scattering (CARS) was proposed for high-resolution 3D sectioning.