Journal ArticleDOI
Alternative bioseparation operations: life beyond packed-bed chromatography.
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The current state of unit operations posing as chromatography alternatives--including membrane filtration, aqueous two-phase extraction, three-phase partitioning, precipitation, crystallization, monoliths and membrane chromatography--and their potential to do the unthinkable are reviewed.About:
This article is published in Current Opinion in Biotechnology.The article was published on 2004-10-01. It has received 325 citations till now. The article focuses on the topics: Downstream processing.read more
Citations
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Recovery and purification process development for monoclonal antibody production
TL;DR: Current methodology used in recovery processes for therapeutic monoclonal antibodies are reviewed here and platform approaches to purification methods development, use of high throughput screening methods, and a view on future developments in purification methodology are offered.
Journal ArticleDOI
Recent Advances in Large-Scale Production of Monoclonal Antibodies and Related Proteins
Abhinav A. Shukla,Jörg Thömmes +1 more
TL;DR: The current state-of-the-art industrial production processes, focusing on downstream technologies, for antibodies and antibody-related products are reviewed and future avenues for evolution are discussed.
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Future of antibody purification.
TL;DR: A wide survey of technologies that are competing to be part of a platform, and an assessment of those that have the most promise are provided.
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Aqueous two-phase systems for protein separation: phase separation and applications.
TL;DR: This paper analyses and reviews some elements that are important for implementation of these processes which are related to phase separation and continuous processing of ATPS.
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Radiation-grafted copolymers for separation and purification purposes: Status, challenges and future directions
TL;DR: In this article, the current status of research on the development and applications of radiation-grafted copolymers for separation and purification purposes is presented without neglecting the seminal work that laid the foundation to today's progress.
References
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Purification of recombinant proteins by fusion with thermally-responsive polypeptides
Dan E. Meyer,Ashutosh Chilkoti +1 more
TL;DR: The ability to environmentally modulate the physicochemical properties of recombinant proteins by fusion with ELPs will allow diverse applications in bioseparation, immunoassays, biocatalysis, and drug delivery.
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Protein separation using membrane chromatography: opportunities and challenges.
TL;DR: The current state of development in the area of membrane chromatographic separation of proteins is reviewed and the technical challenges facing membrane chromatography are highlighted and the scope for future work is discussed.
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Membrane separations in biotechnology
Robert van Reis,Andrew L. Zydney +1 more
TL;DR: Improvements in membrane technology are now focused on high-resolution applications, including improved protein-virus separation, protein purification by high-performance tangential flow filtration and enhanced membrane chromatography, which will allow membranes to play an important role in the evolution of the next generation of biotechnology processes.
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Receptor and transport properties of imprinted polymer membranes – a review
TL;DR: The nature of selectivity of microporous MIP membranes is discussed and a descriptive model for transport selectivity via specific “gates” is developed, which open a way to the design of supramolecular devices that could perform highly selective functions such as recognition, transformation, transfer, regulation and allow signal and information processing.
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Membrane ion-exchange chromatography for process-scale antibody purification.
TL;DR: Cation-exchange membranes in a flow-through mode may provide a reasonable alternative to columns for the removal of low levels of impurities such as DNA, host cell protein, and virus in recombinant monoclonal antibodies.