Journal ArticleDOI
Amino acid analysis : Hydrolysis, ion-exchange cleanup, derivatization, and quantitation by gas—liquid chromatography
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TLDR
The use of ultrasonication to remove dissolved air while pulling a vaccumm on the sample solution prior to hydrolysis assured a good recovery for methionine and cystine as discussed by the authors.About:
This article is published in Journal of Chromatography A.The article was published on 1974-07-17. It has received 365 citations till now. The article focuses on the topics: Sample preparation & Hydrolysis.read more
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Nonparametric Statistics for Non-Statisticians: A Step-by-Step Approach
TL;DR: In this article, the authors present a nonparametric statistical procedure for the test of sample normality in the context of practice questions, and compare the results of two related tests: the Mann-Whitney U -Test and the Fisher Exact Test.
Journal ArticleDOI
Scrapie prion protein contains a phosphatidylinositol glycolipid
TL;DR: Observations indicate that PrPC is anchored to the cell surface by the glycolipid, which is derived from PrPSc by limited proteolysis at the amino terminus.
Journal ArticleDOI
Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves
TL;DR: In this article, 17 fatty acids were observed with α-Linolenic acid (44.57%) having the highest value followed by heneicosanoic (14.41%), g-linolenic (0.20%), palmiteic ( 0.17%), and capric acid (0.,07%), while total polyphenols were 2.02%.
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Nutrional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves
TL;DR: In this article, Moringa oleifera leaves were extracted and unextracted from aqueous ethanol and the true protein contents of these leaves were 93.8% and 81.1% respectively.
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Chemical composition, toxic/antimetabolic constituents, and effects of different treatments on their levels, in four provenances of Jatropha curcas L. from Mexico
TL;DR: In this article, four provenances of J. curcas from different agro-climatic regions of Mexico (1. Castillo de Teayo, 2. Pueblillo 3. Coatzacoalcos and 4. Yautepec), that differed in morphological characteristics, were studied.
References
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Book ChapterDOI
[117] Chromatographic determination of amino acids by the use of automatic recording equipment
Stanford Moore,William H. Stein +1 more
TL;DR: This chapter describes the chromatographic determination of amino acids by the use of automatic recording equipments and suggests that air-oxidation of cysteine in the hydrolyzate to cystine, before chromatography, is desirable in order to obtain a more accurate value for the total cyStine + cySteine content of the protein.
Journal ArticleDOI
Hydrolysis of proteins with p-toluenesulfonic acid. Determination of tryptophan.
Teh-Yung Liu,Y.H. Chang +1 more
TL;DR: The values of tryptophan obtained were close to the expected integral values and the recoveries of all other amino acids were comparable to those observed after hydrolysis with 6 n HCl.
Journal ArticleDOI
Direct esterification of the protein amino acids gas-liquid chromatography of N-TFA n-butyl esters
Don Roach,Charles W. Gehrke +1 more
TL;DR: Gas-liquid chromatography studies of direct esterification of protein amino acids to n-butyl esters have been carried out by as mentioned in this paper for protein amino acid amino acid synthesis.
Journal ArticleDOI
The complete gas—liquid chromatographic separation of the twenty protien amino acids
TL;DR: A new column packing has been found to replace the OV-17 and this is a superior packing and shows quantitative elution, and highly efficient and complete separation of histidine, internal standard tranexamic acid, lysine, arginine, tryptophan, n-butyl stearate, and cystine.
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Gas-liquid chromatography of N-heptafluorobutyryl isobutyl esters of amino acids
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